Product name

Protein A ELISA Kit

Detection method: colourimetric

Example type: Cell culture supernatant

Essay type: sandwich (quantitative)

Sensitivity: 3.7pg/mL

Distance: 7.8pg/mL – 500pg/mL

Rehearsal time: 1h 30m

Test duration: one step test

Product description

The Protein A ELISA Kit (ab215542) is a 90 min single wash sandwich ELISA designed for the quantitative measurement of Protein A protein in the cell culture supernatant. It uses our patented SimpleStep ELISA® technology. Measure Protein A with a sensitivity of 3.7 pg/mL.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This sandwich ELISA approach allows for the formation of the sandwich antibody-analyte complex in a single step, significantly reducing assay time. See the simple steps ELISA® protocol summary in the images section for more details. Our SimpleStep ELISA® technology provides several benefits:

• Unique wash protocol reduces assay time to 90 minutes or less
• High sensitivity, specificity and reproducibility of superior antibodies
• Fully validated in biological samples
• 96-well plate breakable into 12 x 8-well strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available for use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Grades

Protein A (Staphylococcus aureus Protein A) is a 42 kDa protein that is characterized by its ability to bind to the Fc portion of immunoglobulins. Abcam has not applied and does not intend to apply for REACH Authorization of customer uses of products containing substances on the European Authorization list (Annex XIV). It is the responsibility of our customers to verify the need to apply the REACH authorization and any other authorization relevant to the intended uses.

Platform

Precoated microplate (12 x 8-well strips)

Properties

Storage Instructions: Store at +4°C. Check the protocols.

Relevance

Protein A is a surface protein of S. aureus that binds IgG molecules via its Fc region. In serum, bacteria will bind IgG molecules in the wrong orientation on their surface, making opsonization and phagocytosis difficult. Mutants of S. aureus that lack protein A phagocytize more efficiently in vitro, and mutants in infection models have decreased virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Cell localization: secreted; cellular wall.

Alternative names

• gG-binding protein A
• Immunoglobulin G binding protein A
• SPA
• Staphylococcal protein A