Regolazione dell’adrenalina e del glucosio della sintesi e della secrezione di leptina in un pesce teleosteo, la tilapia (Oreochromis mossambicus)

Lo stress acuto è regolato dall’asse simpatico adrenergico in cui le catecolamine mobilitano i negozi di energia insieme ai carboidrati come fattore principale della risposta allo stress endocrino. La leptina  è una citochina vitale per la regolazione del dispendio energetico nei vertebrati ed è stimolata da numerosi fattori di stress nei pesci equivalenti al digiuno, al problema dell’iperosmosi e all’ipossia.
 
Tuttavia, si pensa poco alle interazioni regolatorie tra  leptina  e l’asse dello stress endocrino nei pesci e nei diversi vertebrati ectotermi. Abbiamo valutato le azioni dell’adrenalina e del glucosio nella regolazione della  leptina  A (LepA) in vivo e in vitro nella tilapia. Utilizzando le incubazioni degli epatociti e un omologo LepA  ELISA , presentiamo che la sintesi e la secrezione di LepA diminuiscono con l’aumentare degli intervalli di glucosio ambientale (10-25 mM).
 
Al contrario, la somministrazione di glucosio in bolo nella tilapia aumenterà gli intervalli di mRNA di lepa di 14 volte a 6 ore, suggerendo che elementi sistemici regolati dal glucosio potrebbero contrastare i risultati inibitori diretti del glucosio sull’mRNA di lepa epatico osservati in vitro. L’adrenalina ha stimolato la secrezione di glucosio e LepA dagli epatociti con un andamento dose-dipendente entro 15 minuti, tuttavia ha avuto un impatto limitato sui range di mRNA di lepa. Un’iniezione in vivo di epinefrina nella tilapia ha stimolato un rapido aumento della glicemia che è stato adottato da un aumento di 4 volte negli intervalli di mRNA di lepa epatico a 2,5 e 6 ore. La LepA plasmatica è stata inoltre aumentata di 6 ore rispetto ai controlli. Tilapia ricombinante La somministrazione di LepA in vivo non ha avuto alcun impatto importante sui range di epinefrina plasmatica.
 
I risultati di questa ricerca mostrano che la LepA è regolata negativamente dall’aumento del glucosio extracellulare sullo stadio dell’epatocita, tuttavia stimolato dall’iperglicemia in vivo. Inoltre, l’adrenalina aumenterà la LepA. Questo, insieme al lavoro precedente che dimostra un impatto iperglicemico e glicogenolitico della LepA nella tilapia, significa che l’adrenalina potrebbe stimolare la   secrezione di leptina per migliorare e ottimizzare la mobilizzazione del glucosio e l’omeostasi come parte della risposta allo stress incorporata e adattativa.

La leptina  modula l’espressione genica all’interno del cuore coronarico e dei cardiomiociti nella direzione di mitigare il danno indotto dall’ischemia

La leptina , un ormone della sazietà derivato dagli adipociti, è stata precedentemente collegata alla cardioprotezione. Ora abbiamo dimostrato prima che la  leptina  conferisse resistenza al danno ischemico all’interno del cuore coronarico in topi transgenici αMUPA a lunga vita che sovraesprimono la  leptina  rispetto ai topi con gestione FVB/N di tipo selvatico (WT).
 
Per percepire il contributo della  leptina  al cuore coronarico ischemico, abbiamo misurato proprio qui l’espressione dei geni che codificano per la  leptina  e le proteine ​​​​correlate all’ischemia nei topi αMUPA e WT all’interno del cuore coronarico rispetto al tessuto adiposo dopo infarto miocardico. Inoltre, esaminiamo l’espressione genica nei cardiomiociti neonatali di ratto al di sotto dell’ipossia in assenza e presenza di leptina aggiunta esogenamente   o di un   antagonista della leptina . Abbiamo usato RT-PCR in tempo reale e   saggi ELISA o Western blot per misurare, rispettivamente, i range di mRNA e proteine.
 
I risultati hanno dimostrato che gli  intervalli di leptina circolanti  e gli intervalli di mRNA di  leptina  ed eme ossigenasi-1 (HO-1) all’interno del cuore coronarico sono stati elevati in ciascun genotipo di topo dopo 24 ore di infarto miocardico (MI), raggiungendo valori maggiori nei topi αMUPA. Al contrario,   l’espressione genica della leptina nel tessuto adiposo era notevolmente elevata solo nei topi WT, raggiungendo tuttavia livelli di riduzione rispetto all’intestino. L’espressione dei geni proinfiammatori che codificano per TNFα, IL-1β è stata inoltre ampiamente elevata dopo MI all’interno del cuore coronarico in ciascuna varietà di topi, raggiungendo comunque intervalli significativamente ridotti nei topi αMUPA che indicano uno stato infiammatorio mitigato.
 
Nei cardiomiociti, gli intervalli di mRNA di tutti i geni sopra menzionati oltre ai geni HIF-1α e SOD2 sono stati elevati dopo l’ipossia. Il pretrattamento con leptina esogena ha   ampiamente abbassato gli intervalli di mRNA di TNFα e IL-1β dopo l’ipossia, migliorando l’espressione di tutti i diversi geni e diminuendo gli intervalli di ROS. Il pretrattamento delle cellule con un   antagonista della leptina ha aumentato esclusivamente i gradi di  mRNA della  leptina , suggerendo una regolazione sfavorevole dell’ormone sull’espressione del suo gene personale.
 
In totale, i risultati hanno dimostrato che la  leptina  influisce sull’espressione dei geni nei cardiomiociti al di sotto dell’ipossia in un modo che potrebbe mitigare l’irritazione e lo stress ossidativo, suggerendo lo stesso effetto della leptina endogena   nei topi αMUPA. Inoltre, la  leptina ha  maggiori probabilità di funzionare nel cuore ischemico murino con maggiore successo in un autocrino rispetto al metodo paracrino. Questi risultati suggeriscono che la  leptina può ridurre il danno ischemico modulando l’espressione genica all’interno del cuore coronarico.

Prova di una relazione tra leptina plasmatica  , non Nesfatin-1, e desiderio nei maschi alcoldipendenti dopo l’astinenza.

Lo scopo di questa ricerca era scoprire se la leptina plasmatica  , la nesfatin-1, il cortisolo, il problema neurotrofico di origine cerebrale (BDNF) e le citochine infiammatorie potrebbero essere utilizzati come potenziali biomarcatori per il diploma di craving all’interno del gruppo alcol-dipendente. malati dopo 1 mese di astinenza.
 
Sono stati valutati un totale di 83 pazienti con disfunzione da uso di alcol (AUD) e 61 argomenti salutari. I malati di AUD sono stati scelti dalla Divisione Dipendenza dai Materiali, Anhui Benessere Psicologico Cuore, e gli argomenti all’interno del gruppo di gestione sono stati scelti da volontari sani.
 
La scala del questionario sull’urgenza dell’alcol (AUQ) è stata utilizzata per giudicare l’entità del desiderio per l’alcol, e il controllo di screening dell’alcolismo del Michigan (MAST), il controllo di Fagerstrom per la dipendenza da nicotina (FTND), la scala di valutazione del nervosismo (SAS) e la Self-Score Despair Scale (SDS) è stata inoltre valutata in pazienti con AUD. Il saggio ELISA (Enzyme-Linked Immunosorbent Assay ) è stato utilizzato per la misurazione della leptina plasmatica  , della nesfatin-1, del cortisolo, del BDNF, dell’interleuchina-6 (IL-6), della proteina C-reattiva (CRP) e del fattore di necrosi tumorale-α (TNF). -α) intervalli. Esaminare con controlli sani, la leptina comune  ,  leptina /BMI, IL-6,Gli intervalli di PCR e TNF-α in chi soffre di AUD sono stati considerevolmente elevati, mentre gli intervalli di BDNF sono stati notevolmente ridotti.
 
Inoltre, la valutazione della correlazione parziale ha confermato che i punteggi AUQ dei soggetti alcoldipendenti sono stati correlati positivamente con i  range di leptina plasmatica  ( r  = 0,613,  P  <0,001), relativamente alla nesfatina-1 ( r  = 0,066,  P  = 0,569) dopo aver controllato l’età come covariata. Inoltre, è stato scoperto che gli intervalli plasmatici di nesfatin-1 sono correlati con i punteggi SDS ( r  = 0,366,  P  = 0,001) all’interno del gruppo AUD. Inoltre, gli  intervalli di leptina plasmatica  sono stati correlati positivamente con IL-6 plasmatico ( r  = 0,257,  P  = 0,033), CRP ( r  = 0,305, P  = 0,011) e TNF-α ( r  = 0,311,  P  = 0,009) e correlata negativamente agli intervalli BDNF ( r  = -0,245,  P  = 0,042) in chi soffre di AUD.
 
Questi risultati suggeriscono che la leptina plasmatica  , ma non la nesfatin-1, possa essere un possibile biomarker per il diploma di craving in soggetti alcoldipendenti dopo 1 mese di astinenza, il cui meccanismo può essere associato alla disfunzione delle citochine infiammatorie e del BDNF intervalli.
 
Regolazione dell'adrenalina e del glucosio della sintesi e della secrezione di leptina in un pesce teleosteo, la tilapia (Oreochromis mossambicus)
 

Elevati intervalli di circolazione dell’attivatore del plasminogeno di tipo tissutale sono correlati alla minaccia di aborto spontaneo durante il primo trimestre di gravidanza.

Leptin (Human) ELISA Kit

EKA51863-96T 96T
EUR 446.9

Leptin (human) ELISA Kit

K2191-96 96 tests
EUR 876
Description: Sensitive, Colorimetric Assay

Human Leptin R(Leptin receptor) ELISA Kit

EH0217 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-48T 48T
EUR 396.9

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-5x96T 5x96T
EUR 2693.25

Human Leptin R(Leptin receptor) ELISA Kit

EKF57080-96T 96T
EUR 567

Human Leptin ELISA kit

55R-1635 1 kit
EUR 571.2
Description: ELISA kit for the detection of Human Leptin in the research laboratory

Human Leptin ELISA kit

55R-1998 96 tests
EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA kit

55R-2265 96 tests
EUR 1099.2
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA Kit

DEIA1165 96T
EUR 878.4
Description: The Human LEP ELISA kit is designed to detect and quantify the level of Human LEP in cell culture supernatant, serum, plasma and tissue. This kit is for research use only and not intended for diagnostic purposes.

Human Leptin ELISA Kit

E-80LPT 1 x 96 well plate
EUR 486

Human Leptin ELISA kit

E01L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

E01L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

E01L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

LF-EK50217 1×96T
EUR 777.6

Human Leptin ELISA Kit

EHL0045 96Tests
EUR 625.2

Human Leptin ELISA Kit

RK00035 96 Tests
EUR 625.2

Human Leptin ELISA kit

E01A5566 96T
EUR 700
Description: ELISA

Human Leptin ELISA Kit

IHULEPKT each
EUR 702
Description: Human Leptin ELISA Kit

Human Leptin ELISA Kit

E28L0038 96T
EUR 666.67

Human Leptin ELISA Kit

E16HE0053 96T
EUR 733.33

Human Leptin ELISA KIT

E16HE0053-048 48 wells
EUR 295

Human Leptin ELISA KIT

E16HE0053-096 96 wells
EUR 395

Human Leptin ELISA kit

E22-HC110.48 48T
EUR 295

Human Leptin ELISA kit

E22-HC110.96 96T
EUR 395

Human Leptin ELISA KIT

E42EH-73 96T/48T Ask for price

QuickDetect? Leptin (Human) ELISA Kit

E4449-100 each
EUR 705.6

Camel Leptin(Leptin) Elisa Kit

EK765057 96 Wells
EUR 0.96

Human Leptin (LEP) ELISA Kit

20-abx152192
  • EUR 6448.80
  • EUR 3441.60
  • EUR 811.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Leptin (LEP) ELISA Kit

abx251497-96tests 96 tests
EUR 801.6

Human Leptin,LEP ELISA kit

201-12-1560 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Leptin (LEP) ELISA Kit

abx050142-96tests 96 tests
EUR 801.6

Human Leptin (LEP) ELISA Kit

abx051330-96tests 96 tests
EUR 801.6

Human Leptin (LEP) ELISA Kit

abx570296-96tests 96 tests
EUR 801.6

Human Leptin, LEP ELISA kit

CSB-E04649h-24T 1 plate of 24 wells
EUR 198
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Leptin, LEP ELISA kit

1-CSB-E04649h
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Leptin, LEP ELISA Kit

DEIA167 96T
EUR 888
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitative measurement of Human leptin in serum, plasma and other biological fluids.

Human Leptin,LEP ELISA kit

CN-03239H1 96T
EUR 530.4

Human Leptin,LEP ELISA kit

CN-03239H2 48T
EUR 351.6

Human Leptin (LEP) ELISA Kit

DLR-LEP-Hu-48T 48T
EUR 470.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin (LEP) ELISA Kit

DLR-LEP-Hu-96T 96T
EUR 602.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin(LEP)ELISA Kit

GA-E1576HM-48T 48T
EUR 346.8

Human Leptin(LEP)ELISA Kit

GA-E1576HM-96T 96T
EUR 559.2

Human LEP/ Leptin ELISA Kit

E1447Hu 1 Kit
EUR 644.4

Human LEP(Leptin) ELISA Kit

EH0216 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human Leptin,LEP ELISA Kit

SL1052Hu 96 Tests
EUR 468

Human Leptin, lep ELISA Kit

CSL3505Hu 96 Tests
EUR 468

Human LEP(Leptin) ELISA Kit

EKF57079-48T 48T
EUR 396.9

Human LEP(Leptin) ELISA Kit

EKF57079-5x96T 5x96T
EUR 2693.25

Human LEP(Leptin) ELISA Kit

EKF57079-96T 96T
EUR 567

Human Leptin (LEP) ELISA Kit

EKN53227-48T 48T
EUR 254.38

Human Leptin (LEP) ELISA Kit

EKN53227-5x96T 5x96T
EUR 1726.15

Human Leptin (LEP) ELISA Kit

EKN53227-96T 96T
EUR 363.4

Human Leptin (LEP) ELISA Kit

EKN53228-48T 48T
EUR 277.55

Human Leptin (LEP) ELISA Kit

EKN53228-5x96T 5x96T
EUR 1883.38

Human Leptin (LEP) ELISA Kit

EKN53228-96T 96T
EUR 396.5

Human Leptin (LEP) ELISA Kit

EKU05593-48T 48T
EUR 395.36

Human Leptin (LEP) ELISA Kit

EKU05593-5x96T 5x96T
EUR 2682.8

Human Leptin (LEP) ELISA Kit

EKU05593-96T 96T
EUR 564.8

Human Leptin (LEP) ELISA Kit

RK10066 96T
EUR 280

Human Leptin (LEP) ELISA Kit

EKN46704-48T 48T
EUR 269.71

Human Leptin (LEP) ELISA Kit

EKN46704-5x96T 5x96T
EUR 1830.18

Human Leptin (LEP) ELISA Kit

EKN46704-96T 96T
EUR 385.3

Human Leptin (LEP) ELISA Kit

EKU12117-48T 48T
EUR 408.8

Human Leptin (LEP) ELISA Kit

EKU12117-5x96T 5x96T
EUR 2774

Human Leptin (LEP) ELISA Kit

EKU12117-96T 96T
EUR 584

Human Leptin (LEP) ELISA Kit

EKU12118-48T 48T
EUR 446.11

Human Leptin (LEP) ELISA Kit

EKU12118-5x96T 5x96T
EUR 3027.18

Human Leptin (LEP) ELISA Kit

EKU12118-96T 96T
EUR 637.3

Human Leptin(Lep)ELISA Kit

NSL1884Hu 96 wells
EUR 468

Human LEP(Leptin) ELISA Kit

ELK1160-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Human LEP(Leptin) ELISA Kit

ELK1160-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Leptin (LEP) ELISA Kit

RDR-LEP-Hu-48Tests 48 Tests
EUR 475.2

Human Leptin (LEP) ELISA Kit

RDR-LEP-Hu-96Tests 96 Tests
EUR 654

Human Leptin (LEP) ELISA Kit

AE58414HU-48Tests 48 Tests
EUR 200
Description: Human (Homo sapiens)

Human Leptin (LEP) ELISA Kit

AE58414HU-96Tests 96 Tests
EUR 360
Description: Human (Homo sapiens)

Human Leptin(LEP)ELISA Kit

QY-E01495 96T
EUR 433.2

Human Leptin (LEP) ELISA Kit

RD-LEP-Hu-48Tests 48 Tests
EUR 456

Human Leptin (LEP) ELISA Kit

RD-LEP-Hu-96Tests 96 Tests
EUR 625.2

Human Leptin (LEP) ELISA Kit

RDR-LEP-Hu-48T 48T
EUR 333.17
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Leptin (LEP) ELISA Kit

RDR-LEP-Hu-96T 96T
EUR 475.97
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Leptin (LEP) ELISA Kit

DL-LEP-Hu 96T
EUR 309
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin (LEP) ELISA Kit

DLR-LEP-Hu 96T
EUR 325
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin,LEP ELISA kit

YLA1318HU-48T 48T
EUR 435

Human Leptin,LEP ELISA kit

YLA1318HU-96T 96T
EUR 562.5

Human Leptin (LEP) ELISA Kit

RD-LEP-Hu-48T 48T
EUR 317.3
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Leptin (LEP) ELISA Kit

RD-LEP-Hu-96T 96T
EUR 453.3
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Leptin (LEP) ELISA Kit

SEA084Hu-10x96wellstestplate 10x96-wells test plate
EUR 3855.67
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

SEA084Hu-1x48wellstestplate 1x48-wells test plate
EUR 422.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

SEA084Hu-1x96wellstestplate 1x96-wells test plate
EUR 552.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

SEA084Hu-5x96wellstestplate 5x96-wells test plate
EUR 2125.94
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

4-SEA084Hu
  • EUR 3916.80
  • EUR 2066.40
  • EUR 553.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human LEP (Leptin) ELISA Kit

EKE60107-5x96T 5x96T
EUR 2667.6

Human LEP (Leptin) ELISA Kit

EKE60107-96T 96T
EUR 561.6

Human Leptin,LEP ELISA kit

EKC40091-48T 48T
EUR 328.86

Human Leptin,LEP ELISA kit

EKC40091-5x96T 5x96T
EUR 2231.55

Human Leptin,LEP ELISA kit

EKC40091-96T 96T
EUR 469.8

Human LEP(Leptin) ELISA Kit

E39EH0216 96T
EUR 595

Human LEP(Leptin) ELISA Kit

RE2739H-48wells 48 wells
EUR 125.55

Human LEP(Leptin) ELISA Kit

RE2739H-96wells 96 wells
EUR 179.55

Leptin R ELISA KIT|Human

EF007100 96 Tests
EUR 826.8

Human Leptin R ELISA Kit

E28L0485 96T
EUR 666.67

ELISA kit for Human Leptin

KET6026-48T 48T
EUR 242.4
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin

KET6026-5platesof96wells 5 plates of 96 wells
EUR 1292.4
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin

KET6026-96T 96T
EUR 346.8
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Leptin ELISA kit (4X96T)

LF-EK50218 4×96T
EUR 2641.2

Human Leptin Antibody ELISA kit

E01L0039-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Antibody ELISA kit

E01L0039-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Antibody ELISA kit

E01L0039-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit

E01L0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit

E01L0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit

E01L0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Receptor ELISA Kit

ELA-E0961h 96 Tests
EUR 988.8

Human Leptin PicoKine ELISA Kit

EK0437 96 wells
EUR 510
Description: For quantitative detection of human Leptin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Human Leptin Antibody ELISA kit

E01A5567 96T
EUR 700
Description: ELISA

Human Leptin receptor ELISA kit

E01A5628 96T
EUR 700
Description: ELISA

Leptin ELISA Kit (Human) (OKBB00206)

OKBB00206 96 Tests
EUR 606
Description: Description of target: Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 10 pg/mL

ELISA kit for Human LEP (Leptin)

E-EL-H0113 1 plate of 96 wells
EUR 452.4
Description: A sandwich ELISA kit for quantitative measurement of Human LEP (Leptin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human LEP (Leptin)

ELK1160 1 plate of 96 wells
EUR 446.4
Description: A sandwich ELISA kit for detection of Leptin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human Leptin,LEP

EK0247 96 tests
EUR 723.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Leptin,LEP in samples from serum, plasma, tissue homogenates and other biological fluids.

Nori® Human Leptin ELISA Kit

GR111257 96-well
EUR 461

Human Leptin Flow Cytometry Assay - Group 4

HX88681 96 Tests
EUR 270

Leptin R ELISA Kit| Mouse Leptin Receptor ELISA Kit

EF012890 96 Tests
EUR 826.8

Human Leptin AssayLite Antibody (RPE Conjugate)

11411-05051 150 ug
EUR 513.6

Human Leptin AssayLite Antibody (APC Conjugate)

11411-05061 150 ug
EUR 513.6

EliKine™ Human Leptin ELISA Kit

KTE6026-48T 48 T
EUR 189
Description: EliKine™ Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Human Leptin .

EliKine™ Human Leptin ELISA Kit

KTE6026-96T 96 T
EUR 289
Description: EliKine™ Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Human Leptin .

Human Leptin ELISA

KT-53225 96 tests
EUR 1013

Human Leptin AssayLite Antibody (FITC Conjugate)

11411-05041 150 ug
EUR 513.6

Human Leptin receptor(LR) Elisa Kit

EK710058 96 Wells
EUR 0.57

Mouse Leptin R(Leptin Receptor) ELISA Kit

EM0130 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Human Leptin Receptor (LEPR) ELISA Kit

20-abx152193
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Leptin Receptor (LEPR) ELISA Kit

abx251508-96tests 96 tests
EUR 904.8

Human Leptin Receptor,LEPR ELISA Kit

201-12-0234 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

abx050144-96tests 96 tests
EUR 801.6

Human Leptin Receptor (LEPR) ELISA Kit

abx052393-96tests 96 tests
EUR 801.6

Human Leptin Receptor (LEPR) ELISA Kit

abx573466-96tests 96 tests
EUR 904.8

Human Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Hu-48T 48T
EUR 574.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin Receptor (LEPR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Hu-96T 96T
EUR 745.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin Receptor (LEPR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Leptin receptor(LEPR) ELISA kit

E01L0327-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor(LEPR) ELISA kit

E01L0327-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor(LEPR) ELISA kit

E01L0327-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Receptor(LEPR)ELISA Kit

GA-E0250HM-48T 48T
EUR 346.8

Human Leptin Receptor(LEPR)ELISA Kit

GA-E0250HM-96T 96T
EUR 559.2

Human LEPR/ Leptin receptor ELISA Kit

E1448Hu 1 Kit
EUR 644.4

Human Leptin Receptor, LEPR ELISA Kit

SL1049Hu 96 Tests
EUR 468

Human Leptin Receptor (LEPR) ELISA Kit

EKU05598-48T 48T
EUR 500.5

Human Leptin Receptor (LEPR) ELISA Kit

EKU05598-5x96T 5x96T
EUR 3396.25

Human Leptin Receptor (LEPR) ELISA Kit

EKU05598-96T 96T
EUR 715

Human Leptin Receptor (LEPR) ELISA Kit

EKL58736-5x96T 5x96T
EUR 3452.78

Human Leptin Receptor (LEPR) ELISA Kit

EKL58736-96T 96T
EUR 726.9

Human Leptin Receptor (LEPR) ELISA Kit

EKN46709-48T 48T
EUR 341.04

Human Leptin Receptor (LEPR) ELISA Kit

EKN46709-5x96T 5x96T
EUR 2314.2

Human Leptin Receptor (LEPR) ELISA Kit

EKN46709-96T 96T
EUR 487.2

Human Leptin Receptor,LEPR ELISA Kit

YLA0298HU-48T 48T
EUR 435

Human Leptin Receptor,LEPR ELISA Kit

YLA0298HU-96T 96T
EUR 562.5

Human LEPR(Leptin Receptor) ELISA Kit

ELK2281-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEPR in the samples is then determined by comparing the OD of the samples to the standard curve.

Human LEPR(Leptin Receptor) ELISA Kit

ELK2281-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEPR in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Leptin Receptor (LEPR)ELISA Kit

NSL1885Hu 96 wells
EUR 468

Human Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Hu-48Tests 48 Tests
EUR 600

Human Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Hu-96Tests 96 Tests
EUR 830.4

Human Leptin Receptor(LEPR)ELISA Kit

QY-E01494 96T
EUR 433.2

Human Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Hu-48Tests 48 Tests
EUR 573.6

Human Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Hu-96Tests 96 Tests
EUR 794.4

Human Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Hu-48T 48T
EUR 432.39
Description: serum, plasma, tissue homogenates and other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Hu-96T 96T
EUR 617.72
Description: serum, plasma, tissue homogenates and other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

DL-LEPR-Hu 96T
EUR 402
Description: serum, plasma, tissue homogenates or other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Hu 96T
EUR 422
Description: serum, plasma, tissue homogenates or other biological fluids.

Human Leptin Receptor(LEPR) Elisa Kit

EK711119 96 Wells
EUR 0.14

Human Leptin receptor(LEPR) ELISA kit

E01A5671 96T
EUR 700
Description: ELISA

Human Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Hu-48T 48T
EUR 411.8
Description: serum, plasma, tissue homogenates and other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Hu-96T 96T
EUR 588.3
Description: serum, plasma, tissue homogenates and other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

SEA083Hu-10x96wellstestplate 10x96-wells test plate
EUR 5128.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

SEA083Hu-1x48wellstestplate 1x48-wells test plate
EUR 527.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

SEA083Hu-1x96wellstestplate 1x96-wells test plate
EUR 702.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Human Leptin Receptor (LEPR) ELISA Kit

SEA083Hu-5x96wellstestplate 5x96-wells test plate
EUR 2799.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

L’attivatore tissutale del plasminogeno ha confermato una grande distinzione (p = 0,0004) tra ciascuna squadra, sebbene né l’adiponectina né la leptina abbiano rivelato importanti aggiustamenti, indicando che le ragazze che hanno avuto aborti spontanei hanno gamme considerevolmente maggiori di attivatore tissutale del plasminogeno rispetto alle donne che hanno avuto gravidanze regolari.

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