Regolazione dell’adrenalina e del glucosio della sintesi e della secrezione di leptina in un pesce teleosteo, la tilapia (Oreochromis mossambicus)

Lo stress acuto è regolato dall’asse simpatico adrenergico in cui le catecolamine mobilitano i negozi di energia insieme ai carboidrati come fattore principale della risposta allo stress endocrino. La leptina  è una citochina vitale per la regolazione del dispendio energetico nei vertebrati ed è stimolata da numerosi fattori di stress nei pesci equivalenti al digiuno, al problema dell’iperosmosi e all’ipossia.
 
Tuttavia, si pensa poco alle interazioni regolatorie tra  leptina  e l’asse dello stress endocrino nei pesci e nei diversi vertebrati ectotermi. Abbiamo valutato le azioni dell’adrenalina e del glucosio nella regolazione della  leptina  A (LepA) in vivo e in vitro nella tilapia. Utilizzando le incubazioni degli epatociti e un omologo LepA  ELISA , presentiamo che la sintesi e la secrezione di LepA diminuiscono con l’aumentare degli intervalli di glucosio ambientale (10-25 mM).
 
Al contrario, la somministrazione di glucosio in bolo nella tilapia aumenterà gli intervalli di mRNA di lepa di 14 volte a 6 ore, suggerendo che elementi sistemici regolati dal glucosio potrebbero contrastare i risultati inibitori diretti del glucosio sull’mRNA di lepa epatico osservati in vitro. L’adrenalina ha stimolato la secrezione di glucosio e LepA dagli epatociti con un andamento dose-dipendente entro 15 minuti, tuttavia ha avuto un impatto limitato sui range di mRNA di lepa. Un’iniezione in vivo di epinefrina nella tilapia ha stimolato un rapido aumento della glicemia che è stato adottato da un aumento di 4 volte negli intervalli di mRNA di lepa epatico a 2,5 e 6 ore. La LepA plasmatica è stata inoltre aumentata di 6 ore rispetto ai controlli. Tilapia ricombinante La somministrazione di LepA in vivo non ha avuto alcun impatto importante sui range di epinefrina plasmatica.
 
I risultati di questa ricerca mostrano che la LepA è regolata negativamente dall’aumento del glucosio extracellulare sullo stadio dell’epatocita, tuttavia stimolato dall’iperglicemia in vivo. Inoltre, l’adrenalina aumenterà la LepA. Questo, insieme al lavoro precedente che dimostra un impatto iperglicemico e glicogenolitico della LepA nella tilapia, significa che l’adrenalina potrebbe stimolare la   secrezione di leptina per migliorare e ottimizzare la mobilizzazione del glucosio e l’omeostasi come parte della risposta allo stress incorporata e adattativa.

La leptina  modula l’espressione genica all’interno del cuore coronarico e dei cardiomiociti nella direzione di mitigare il danno indotto dall’ischemia

La leptina , un ormone della sazietà derivato dagli adipociti, è stata precedentemente collegata alla cardioprotezione. Ora abbiamo dimostrato prima che la  leptina  conferisse resistenza al danno ischemico all’interno del cuore coronarico in topi transgenici αMUPA a lunga vita che sovraesprimono la  leptina  rispetto ai topi con gestione FVB/N di tipo selvatico (WT).
 
Per percepire il contributo della  leptina  al cuore coronarico ischemico, abbiamo misurato proprio qui l’espressione dei geni che codificano per la  leptina  e le proteine ​​​​correlate all’ischemia nei topi αMUPA e WT all’interno del cuore coronarico rispetto al tessuto adiposo dopo infarto miocardico. Inoltre, esaminiamo l’espressione genica nei cardiomiociti neonatali di ratto al di sotto dell’ipossia in assenza e presenza di leptina aggiunta esogenamente   o di un   antagonista della leptina . Abbiamo usato RT-PCR in tempo reale e   saggi ELISA o Western blot per misurare, rispettivamente, i range di mRNA e proteine.
 
I risultati hanno dimostrato che gli  intervalli di leptina circolanti  e gli intervalli di mRNA di  leptina  ed eme ossigenasi-1 (HO-1) all’interno del cuore coronarico sono stati elevati in ciascun genotipo di topo dopo 24 ore di infarto miocardico (MI), raggiungendo valori maggiori nei topi αMUPA. Al contrario,   l’espressione genica della leptina nel tessuto adiposo era notevolmente elevata solo nei topi WT, raggiungendo tuttavia livelli di riduzione rispetto all’intestino. L’espressione dei geni proinfiammatori che codificano per TNFα, IL-1β è stata inoltre ampiamente elevata dopo MI all’interno del cuore coronarico in ciascuna varietà di topi, raggiungendo comunque intervalli significativamente ridotti nei topi αMUPA che indicano uno stato infiammatorio mitigato.
 
Nei cardiomiociti, gli intervalli di mRNA di tutti i geni sopra menzionati oltre ai geni HIF-1α e SOD2 sono stati elevati dopo l’ipossia. Il pretrattamento con leptina esogena ha   ampiamente abbassato gli intervalli di mRNA di TNFα e IL-1β dopo l’ipossia, migliorando l’espressione di tutti i diversi geni e diminuendo gli intervalli di ROS. Il pretrattamento delle cellule con un   antagonista della leptina ha aumentato esclusivamente i gradi di  mRNA della  leptina , suggerendo una regolazione sfavorevole dell’ormone sull’espressione del suo gene personale.
 
In totale, i risultati hanno dimostrato che la  leptina  influisce sull’espressione dei geni nei cardiomiociti al di sotto dell’ipossia in un modo che potrebbe mitigare l’irritazione e lo stress ossidativo, suggerendo lo stesso effetto della leptina endogena   nei topi αMUPA. Inoltre, la  leptina ha  maggiori probabilità di funzionare nel cuore ischemico murino con maggiore successo in un autocrino rispetto al metodo paracrino. Questi risultati suggeriscono che la  leptina può ridurre il danno ischemico modulando l’espressione genica all’interno del cuore coronarico.

Prova di una relazione tra leptina plasmatica  , non Nesfatin-1, e desiderio nei maschi alcoldipendenti dopo l’astinenza.

Lo scopo di questa ricerca era scoprire se la leptina plasmatica  , la nesfatin-1, il cortisolo, il problema neurotrofico di origine cerebrale (BDNF) e le citochine infiammatorie potrebbero essere utilizzati come potenziali biomarcatori per il diploma di craving all’interno del gruppo alcol-dipendente. malati dopo 1 mese di astinenza.
 
Sono stati valutati un totale di 83 pazienti con disfunzione da uso di alcol (AUD) e 61 argomenti salutari. I malati di AUD sono stati scelti dalla Divisione Dipendenza dai Materiali, Anhui Benessere Psicologico Cuore, e gli argomenti all’interno del gruppo di gestione sono stati scelti da volontari sani.
 
La scala del questionario sull’urgenza dell’alcol (AUQ) è stata utilizzata per giudicare l’entità del desiderio per l’alcol, e il controllo di screening dell’alcolismo del Michigan (MAST), il controllo di Fagerstrom per la dipendenza da nicotina (FTND), la scala di valutazione del nervosismo (SAS) e la Self-Score Despair Scale (SDS) è stata inoltre valutata in pazienti con AUD. Il saggio ELISA (Enzyme-Linked Immunosorbent Assay ) è stato utilizzato per la misurazione della leptina plasmatica  , della nesfatin-1, del cortisolo, del BDNF, dell’interleuchina-6 (IL-6), della proteina C-reattiva (CRP) e del fattore di necrosi tumorale-α (TNF). -α) intervalli. Esaminare con controlli sani, la leptina comune  ,  leptina /BMI, IL-6,Gli intervalli di PCR e TNF-α in chi soffre di AUD sono stati considerevolmente elevati, mentre gli intervalli di BDNF sono stati notevolmente ridotti.
 
Inoltre, la valutazione della correlazione parziale ha confermato che i punteggi AUQ dei soggetti alcoldipendenti sono stati correlati positivamente con i  range di leptina plasmatica  ( r  = 0,613,  P  <0,001), relativamente alla nesfatina-1 ( r  = 0,066,  P  = 0,569) dopo aver controllato l’età come covariata. Inoltre, è stato scoperto che gli intervalli plasmatici di nesfatin-1 sono correlati con i punteggi SDS ( r  = 0,366,  P  = 0,001) all’interno del gruppo AUD. Inoltre, gli  intervalli di leptina plasmatica  sono stati correlati positivamente con IL-6 plasmatico ( r  = 0,257,  P  = 0,033), CRP ( r  = 0,305, P  = 0,011) e TNF-α ( r  = 0,311,  P  = 0,009) e correlata negativamente agli intervalli BDNF ( r  = -0,245,  P  = 0,042) in chi soffre di AUD.
 
Questi risultati suggeriscono che la leptina plasmatica  , ma non la nesfatin-1, possa essere un possibile biomarker per il diploma di craving in soggetti alcoldipendenti dopo 1 mese di astinenza, il cui meccanismo può essere associato alla disfunzione delle citochine infiammatorie e del BDNF intervalli.
 
Regolazione dell'adrenalina e del glucosio della sintesi e della secrezione di leptina in un pesce teleosteo, la tilapia (Oreochromis mossambicus)
 

Elevati intervalli di circolazione dell’attivatore del plasminogeno di tipo tissutale sono correlati alla minaccia di aborto spontaneo durante il primo trimestre di gravidanza.

MicroMolar ADP Assay Kit - 100 assays

MAD100K 100 assays
EUR 278.4
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MAD reagent 1, 30 ul of 100 x MAD reagent 2 and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of ADP using a standard black 384-well plate.

MicroMolar GDP assay kit - 100 assays

MGD100K 100 assays
EUR 278.4
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MGD reagent 1, 30 ul of 100 x MGD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of GDP using a standard black 384-well plate.

MicroMolar UDP assay kit - 100 assays

MUD100K 100 assays
EUR 278.4
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MUD reagent 1, 30 ul of 100 x MUD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of UDP using a standard black 384-well plate.

Leptin (human) ELISA Kit

K4777-100 100 assays
EUR 970.8
Description: Sensitive, Colorimetric Assay

Leptin (human) ELISA Kit

K2191-100 100 assays
EUR 1220.4

Leptin (Human) ELISA Kit

E4653-100 each
EUR 874.8

QuickDetect? Leptin (Human) ELISA Kit

E4449-100 each
EUR 705.6

E. coli BirA - for 100 assays

BIRA-100KEC 100 assays
EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli BirA.

E. coli GlmU - for 100 assays

GLMU-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 500 nM E. coli GlmU.

E. coli gyrA - for 100 assays

GYRA-100EC 100 assays
EUR 246.49
Description: This product includes 50 ul of 2 uM E.coli gyrA

P. aeruginosa gyrA - for 100 assays

GYRA-100PA 100 assays
EUR 246.49
Description: This product includes 50 ul of 2 uM P. aeruginosa gyrA

S. aureus gyrA - for 100 assays

GYRA-100SA 100 assays
EUR 246.49
Description: This product includes 43 ul of 7.5 uM S. aureus gyrA

E. coli gyrB - for 100 assays

GYRB-100EC 100 assays
EUR 246.49
Description: This product includes 50 ul of 2 uM E.coli gyrB

P. aeruginosa gyrB - for 100 assays

GYRB-100PA 100 assays
EUR 246.49
Description: This product includes 50 ul of 2 uM P. aeruginosa gyrB

S. aureus gyrB - for 100 assays

GYRB-100SA 100 assays
EUR 246.49
Description: This product includes 43 ul of 7.5 uM S. aureus gyrB

E. coli IspD - for 100 assays

ISPD-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 200 nM E. coli IspD.

P. aeruginosa IspD - for 100 assays

ISPD-100PA 100 assays
EUR 309.4
Description: This product includes 35 ul of 200 nM P. aeruginosa IspD.

E. coli MurA - for 100 assays

MURA-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli MurA.

E. coli MurC - for 100 assays

MURC-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli MurC.

P. aeruginosa MurC - for 100 assays

MURC-100PA 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM P. aeruginosa MurC.

S. pneumonia MurC - for 100 assays

MURC-100SP 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM S. pneumoniae MurC.

E. coli MurD - for 100 assays

MURD-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 2 uM E. coli MurD.

P. aeruginosa MurD - for 100 assays

MURD-100PA 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM P. aeruginosa MurD.

S. aureus MurD - for 100 assays

MURD-100SA 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM S. aureus MurD.

E. coli MurE - for 100 assays

MURE-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli MurE

E. coli MurF - for 100 assays

MURF-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM E. coli MurF.

P. aeruginosa MurF - for 100 assays

MURF-100PA 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM P. aeruginosa MurF.

S. aureus MurF - for 100 assays

MURF-100SA 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM S. aureus MurF.

S. pneumonia MurF - for 100 assays

MURF-100SP 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM S. pneumoniae MurF.

E. coli parC - for 100 assays

PARC-100EC 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM E. coli parC

P. aeruginosa parC - for 100 assays

PARC-100PA 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM P. aeruginosa parC

S. aureus parC - for 100 assays

PARC-100SA 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM S. aureus ParC

S. pneumoniae parC - for 100 assays

PARC-100SP 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM S. pneumoniae parC

E. coli parE - for 100 assays

PARE-100EC 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM E. coli parE

P. aeruginosa parE - for 100 assays

PARE-100PA 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM P. aeruginosa parE

S. aureus parE - for 100 assays

PARE-100SA 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM S. aureus ParE

S. pneumoniae parE - for 100 assays

PARE-100SP 100 assays
EUR 246.49
Description: This product includes 50 ul of 5 uM S. pneumoniae parE

S. aureus PncB - for 100 assays

PNCB-100SA 100 assays
EUR 309.4
Description: This product includes 35 ul of 5 uM S. aureus PNCB.

S. pneumonia PncB - for 100 assays

PNCB-100SP 100 assays
EUR 309.4
Description: This product includes 35 ul of 5 uM S. pneumoniae PNCB.

Leptin (Mouse) ELISA Kit

E4654-100 each
EUR 874.8

Leptin (Rat) ELISA Kit

E4655-100 each
EUR 874.8

AccuGreen High Sensitivity dsDNA Quantitation Kit (100 assays)

31066-T 100kit
EUR 159.6
Description: Minimum order quantity: 1 unit of 100kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (100 assays)

31069-T 1kit
EUR 148.8
Description: Minimum order quantity: 1 unit of 1kit

Annexin V, R-phycoerythrin conjugate (100 assays)

9-29045
  • EUR 178.80
  • EUR 374.40
  • 100uL
  • 500uL
Description: Minimum order quantity: 1 unit of 500uL

Annexin V, Allophycocyanin crosslinked conjugate (100 assays)

9-29057
  • EUR 178.80
  • EUR 374.40
  • 100uL
  • 500uL
Description: Minimum order quantity: 1 unit of 500uL

E. coli DNA helicase - for 100 assays

DNAB-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM E. coli DNA helicase.

H. influenza DNA helicase - for 100 assays

DNAB-100HI 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM H influenzae DNA helicase.

P. aeruginosa DNA helicase - for 100 assays

DNAB-100PA 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM DNA P. aeruginosa helicase.

S. pneumonia DNA helicase - for 100 assays

DNAB-100SP 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM S. pneumoniae DNA helicase.

E. coli DNA primase - for 100 assays

DNAG-100EC 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM E. coli primase-helicase complex

H. influenza DNA primase - for 100 assays

DNAG-100HI 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM H. influenzae primase-helicase complex

S. aureus DNA primase - for 100 assays

DNAG-100SA 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM S. aureus primase.

S. pneumonia DNA primase - for 100 assays

DNAG-100SP 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM S. pneumoniae primase.

E. coli guanylate kinase - for 100 assays

GMK-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul 1 uM E. coli GMP kinase

S. pneumoniae guanylate kinase - for 100 assays

GMK-100SP 100 assays
EUR 309.4
Description: This product includes 35 ul 1 uM S. pneumoniae GMP kinase

E. coli DNA ligase - for 100 assays

LIG-100EC 100 assays
EUR 309.4
Description: This product includes 10 ul of 500 nM E. coli ligase.

E. coli NAD kinase - for 100 assays

NADK-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 2 uM E. coli UMP kinase

E. coli polynucleotide phosphorylase - for 100 assays

PNP-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 25 uM E. coli PNPase.

E. coli RNA polymerase - for 100 assays

RNAP-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM E.coli RNA Polymerase.

S. aureus RNA polymerase - for 100 assays

RNAP-100SA 100 assays
EUR 399.28
Description: This product includes 33 ul of 2 uM S. aureus RNA Polymerase.

E. coli DNA gyrase - for 100 assays

TOP2-100EC 100 assays
EUR 309.4
Description: This product includes wild-type E. coli gyrase for 100 assays (40 ul of 2000 nM stock solution)

P. aeruginosa DNA gyrase - for 100 assays

TOP2-100PA 100 assays
EUR 309.4
Description: This product includes 50 ul of 2 uM P. aeruginosa gyrase

S. aureus DNA gyrase - for 100 assays

TOP2-100SA 100 assays
EUR 309.4
Description: This product includes 43 ul of 7.5 uM S. aureus gyrase.

E. coli UMP kinase - for 100 assays

UMK-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 1uM E. coli UMP kinase

SARS-Cov-2 RNA polymerase assay kit-100 (100 assays, enzyme not included)

S2RPA100K 100 assays
EUR 556
Description: This product includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x NTPs (ATP, UTP, GTP, CTP) and 700 µl of 10 x fluorescence dye. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format. The assay kit includes all reagents except the enzyme.

SARS-Cov-2 RNA polymerase assay kit plus-100 (100 assays, enzyme included)

S2RPA100KE 100 assays
EUR 1484.8
Description: This product includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x mM NTPs (ATP, UTP, GTP, CTP), 700 µl of 10 x fluorescence dye and 50 µl of 50 x SARS-CoV-2 RdRp. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format.

Human Leptin R(Leptin receptor) ELISA Kit

EH0217 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

AccuGreen High Sensitivity dsDNA Quantitation Solution (100 assays)

31068-T 100kit
EUR 139.2
Description: Minimum order quantity: 1 unit of 100kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (100 assays)

31070-T 1kit
EUR 130.8
Description: Minimum order quantity: 1 unit of 1kit

E. coli DNA polymerase alpha - for 100 assays

DPA-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM E. coli DNA polymerase III alpha.

H. influenza DNA polymerase alpha - for 100 assays

DPA-100HI 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM H. influenzae DNA polymerase III alpha.

S. pneumonia DNA polymerase alpha - for 100 assays

DPA-100SP 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM S. pneumoniae DNA polymerase III alpha.

E. coli thymidylate kinase (TMK) - for 100 assays

TMK-100EC 100 assays
EUR 309.4
Description: This product includes 35 ul of 1uM E. coli Thymidylate kinase

E. coli DNA topoisomerase I - for 100 assays

TOP1-100EC 100 assays
EUR 309.4
Description: This product includes 42 ul of 4 uM E. coli topoisomerase I

E. coli topoisomerase IV holoenzyme - for 100 assays

TOP4-100EC 100 assays
EUR 309.4
Description: This product includes 50 ul of 5 uM E. coli topoisomerase IV (parC-parE)

P. aeruginosa DNA topoisomerase IV - for 100 assays

TOP4-100PA 100 assays
EUR 309.4
Description: This product includes 50 ul of 5 uMP. aeruginosa topoisomerase IV (parC-parE)

S. aureus DNA topoisomerase IV - for 100 assays

TOP4-100SA 100 assays
EUR 309.4
Description: This product includes 50 ul of 5 uM S. aureus DNA topoisomerase IV.

S. pneumoniae DNA topoisomerase IV - for 100 assays

TOP4-100SP 100 assays
EUR 309.4
Description: This product includes 50 ul of 5 uM S. pneumoniae DNA topoisomerase IV

EGTT-Scavenger for GSH/GSSG Assays (EGTT-100)

EGTT-SCVG 100
EUR 87
Description: EGTT-Scavenger (cat# EGTT-SCVG) is a chemical reagent used for sample treatment with the GSH/GSSG Assay Kit (cat#: EGTT-100). Necessary for cell lysate samples used in the EGTT-100 assay kit. Key Features: Ready-to-use 500µL solution, sufficient for 100 tests with EGTT-100 assay kit.

Human Leptin ELISA kit

E01L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

E01L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

E01L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA Kit

DEIA1165 96T
EUR 878.4
Description: The Human LEP ELISA kit is designed to detect and quantify the level of Human LEP in cell culture supernatant, serum, plasma and tissue. This kit is for research use only and not intended for diagnostic purposes.

Human Leptin ELISA Kit

E-80LPT 1 x 96 well plate
EUR 486

Human Leptin ELISA Kit

EHL0045 96Tests
EUR 625.2

Human Leptin ELISA kit

55R-2265 96 tests
EUR 1099.2
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA kit

55R-1635 1 kit
EUR 571.2
Description: ELISA kit for the detection of Human Leptin in the research laboratory

Human Leptin ELISA kit

55R-1998 96 tests
EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA kit

LF-EK50217 1×96T
EUR 777.6

Human Leptin ELISA Kit

RK00035 96 Tests
EUR 625.2

NucView 488 and MitoView 633 Apoptosis Assay Kit (100 assays)

30062 1KIT
EUR 514.8
Description: Minimum order quantity: 1 unit of 1KIT

NucView 488 and RedDot 2 Apoptosis & Necrosis Kit (100 assays)

30072 1KIT
EUR 458.4
Description: Minimum order quantity: 1 unit of 1KIT

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 242.4

Leptin Antibody

5366-100 each
EUR 379.2

Leptin Antibody

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EUR 379.2

Leptin Antibody

5368-100 each
EUR 379.2

Gel-based E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus-100 (for 100 assays)

GDSA100KE 100 assays
EUR 399.28
Description: This product includes 400 ul of 10 x Buffer, 105 ul of 250 ug/ml relaxed DNA, 220 ul of 10 mM ATP, 550 ul of 5 x Gel loading buffer and 25 ul of 2000 nM E.coli gyrase.

Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30008-2 1KIT
EUR 459.6
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30011-2 1KIT
EUR 459.6
Description: Minimum order quantity: 1 unit of 1KIT

Human Leptin Antibody ELISA kit

E01L0039-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Antibody ELISA kit

E01L0039-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Antibody ELISA kit

E01L0039-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin, LEP ELISA kit

CSB-E04649h-24T 1 plate of 24 wells
EUR 198
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Leptin, LEP ELISA kit

1-CSB-E04649h
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Leptin receptor ELISA kit

E01L0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit

E01L0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit

E01L0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin, LEP ELISA Kit

DEIA167 96T
EUR 888
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitative measurement of Human leptin in serum, plasma and other biological fluids.

Human Leptin,LEP ELISA kit

CN-03239H1 96T
EUR 530.4

Human Leptin,LEP ELISA kit

CN-03239H2 48T
EUR 351.6

Human LEP/ Leptin ELISA Kit

E1447Hu 1 Kit
EUR 644.4

Human Leptin (LEP) ELISA Kit

DLR-LEP-Hu-48T 48T
EUR 470.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin (LEP) ELISA Kit

DLR-LEP-Hu-96T 96T
EUR 602.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin PicoKine ELISA Kit

EK0437 96 wells
EUR 510
Description: For quantitative detection of human Leptin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Human Leptin (LEP) ELISA Kit

20-abx152192
  • EUR 6448.80
  • EUR 3441.60
  • EUR 811.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Leptin (LEP) ELISA Kit

abx050142-96tests 96 tests
EUR 801.6

Human Leptin (LEP) ELISA Kit

abx051330-96tests 96 tests
EUR 801.6

Human Leptin (LEP) ELISA Kit

abx570296-96tests 96 tests
EUR 801.6

Human Leptin (LEP) ELISA Kit

abx251497-96tests 96 tests
EUR 801.6

Human Leptin,LEP ELISA kit

201-12-1560 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Leptin ELISA Kit (Human) (OKBB00206)

OKBB00206 96 Tests
EUR 606
Description: Description of target: Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 10 pg/mL

ELISA kit for Human Leptin

KET6026-48T 48T
EUR 242.4
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin

KET6026-5platesof96wells 5 plates of 96 wells
EUR 1292.4
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin

KET6026-96T 96T
EUR 346.8
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Leptin ELISA kit (4X96T)

LF-EK50218 4×96T
EUR 2641.2

Human LEP(Leptin) ELISA Kit

EH0216 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human Leptin AssayMax ELISA Kit

EL2001-1 96 Well Plate
EUR 500.4

Human Leptin Receptor ELISA Kit

ELA-E0961h 96 Tests
EUR 988.8

Human Leptin(LEP)ELISA Kit

GA-E1576HM-48T 48T
EUR 346.8

Human Leptin(LEP)ELISA Kit

GA-E1576HM-96T 96T
EUR 559.2

Leptin R ELISA KIT|Human

EF007100 96 Tests
EUR 826.8

Human Leptin (LEP) ELISA Kit

RD-LEP-Hu-48Tests 48 Tests
EUR 456

Human Leptin (LEP) ELISA Kit

RD-LEP-Hu-96Tests 96 Tests
EUR 625.2

Human Leptin(LEP)ELISA Kit

QY-E01495 96T
EUR 433.2

Human Leptin (LEP) ELISA Kit

RDR-LEP-Hu-48Tests 48 Tests
EUR 475.2

Human Leptin (LEP) ELISA Kit

RDR-LEP-Hu-96Tests 96 Tests
EUR 654

Human Leptin (LEP) ELISA Kit

SEA084Hu-10x96wellstestplate 10x96-wells test plate
EUR 3855.67
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

SEA084Hu-1x48wellstestplate 1x48-wells test plate
EUR 422.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

SEA084Hu-1x96wellstestplate 1x96-wells test plate
EUR 552.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

SEA084Hu-5x96wellstestplate 5x96-wells test plate
EUR 2125.94
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit

4-SEA084Hu
  • EUR 3916.80
  • EUR 2066.40
  • EUR 553.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Leptin,LEP ELISA kit

YLA1318HU-48T 48T
EUR 435

Human Leptin,LEP ELISA kit

YLA1318HU-96T 96T
EUR 562.5

Leptin R ELISA Kit| Mouse Leptin Receptor ELISA Kit

EF012890 96 Tests
EUR 826.8

Human Leptin ELISA Kit, 96 tests, Quantitative, 96 tests

100-135-LEH 1 kit
EUR 781.2

Mouse Leptin ELISA Kit, 96 tests, Quantitative

100-130-LEM 1 Kit
EUR 854.4

Mouse Leptin R(Leptin Receptor) ELISA Kit

EM0130 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Recombinant Human Leptin Triple Antagonist

CG072-100 100ug
EUR 489.6

ViaFluor 488 SE Cell Proliferation Kit, Trial Size (1 vial, 100 assays)

30086-T 1KIT
EUR 124.8
Description: Minimum order quantity: 1 unit of 1KIT

E. coli D-Ala-D-Ala ligase - for 100 assays

DDL-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 200 nM E. coli D-Ala:D-Ala ligase.

Leptin Receptor Antibody

5582-100 each
EUR 405.6

Goat Leptin ELISA kit

E06L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Leptin ELISA kit

E06L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Leptin ELISA kit

E06L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Leptin ELISA kit

E08L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Leptin ELISA kit

E08L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Leptin ELISA kit

E08L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit

E04L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit

E04L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit

E04L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Leptin ELISA kit

E07L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Leptin ELISA kit

E07L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Leptin ELISA kit

E07L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit

E03L0038-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit

E03L0038-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit

E03L0038-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA Kit

E-90LPT 1 x 96 well plate
EUR 529.2

Mouse Leptin ELISA Kit

EM0129 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 37.5pg/ml

Sheep Leptin ELISA Kit

ESL0045 96Tests
EUR 625.2

Porcine Leptin ELISA Kit

EPL0045 96Tests
EUR 625.2

Monkey Leptin ELISA Kit

EMKL0045 96Tests
EUR 625.2

Mouse Leptin ELISA Kit

EML0045 96Tests
EUR 625.2

Rat Leptin ELISA Kit

ERL0045 96Tests
EUR 625.2

L’attivatore tissutale del plasminogeno ha confermato una grande distinzione (p = 0,0004) tra ciascuna squadra, sebbene né l’adiponectina né la leptina abbiano rivelato importanti aggiustamenti, indicando che le ragazze che hanno avuto aborti spontanei hanno gamme considerevolmente maggiori di attivatore tissutale del plasminogeno rispetto alle donne che hanno avuto gravidanze regolari.

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