Regolazione dell’adrenalina e del glucosio della sintesi e della secrezione di leptina in un pesce teleosteo, la tilapia (Oreochromis mossambicus)

Lo stress acuto è regolato dall’asse simpatico adrenergico in cui le catecolamine mobilitano i negozi di energia insieme ai carboidrati come fattore principale della risposta allo stress endocrino. La leptina è una citochina vitale per la regolazione del dispendio energetico nei vertebrati ed è stimolata da numerosi fattori di stress nei pesci equivalenti al digiuno, al problema dell’iperosmosi e all’ipossia.

Tuttavia, si pensa poco alle interazioni regolatorie tra leptina e l’asse dello stress endocrino nei pesci e nei diversi vertebrati ectotermi. Abbiamo valutato le azioni dell’adrenalina e del glucosio nella regolazione della leptina A (LepA) in vivo e in vitro nella tilapia. Utilizzando le incubazioni degli epatociti e un omologo LepA ELISA , presentiamo che la sintesi e la secrezione di LepA diminuiscono con l’aumentare degli intervalli di glucosio ambientale (10-25 mM).

Al contrario, la somministrazione di glucosio in bolo nella tilapia aumenterà gli intervalli di mRNA di lepa di 14 volte a 6 ore, suggerendo che elementi sistemici regolati dal glucosio potrebbero contrastare i risultati inibitori diretti del glucosio sull’mRNA di lepa epatico osservati in vitro. L’adrenalina ha stimolato la secrezione di glucosio e LepA dagli epatociti con un andamento dose-dipendente entro 15 minuti, tuttavia ha avuto un impatto limitato sui range di mRNA di lepa. Un’iniezione in vivo di epinefrina nella tilapia ha stimolato un rapido aumento della glicemia che è stato adottato da un aumento di 4 volte negli intervalli di mRNA di lepa epatico a 2,5 e 6 ore. La LepA plasmatica è stata inoltre aumentata di 6 ore rispetto ai controlli. Tilapia ricombinante La somministrazione di LepA in vivo non ha avuto alcun impatto importante sui range di epinefrina plasmatica.

I risultati di questa ricerca mostrano che la LepA è regolata negativamente dall’aumento del glucosio extracellulare sullo stadio dell’epatocita, tuttavia stimolato dall’iperglicemia in vivo. Inoltre, l’adrenalina aumenterà la LepA. Questo, insieme al lavoro precedente che dimostra un impatto iperglicemico e glicogenolitico della LepA nella tilapia, significa che l’adrenalina potrebbe stimolare la secrezione di leptina per migliorare e ottimizzare la mobilizzazione del glucosio e l’omeostasi come parte della risposta allo stress incorporata e adattativa.

La leptina modula l’espressione genica all’interno del cuore coronarico e dei cardiomiociti nella direzione di mitigare il danno indotto dall’ischemia

La leptina , un ormone della sazietà derivato dagli adipociti, è stata precedentemente collegata alla cardioprotezione. Ora abbiamo dimostrato prima che la leptina conferisse resistenza al danno ischemico all’interno del cuore coronarico in topi transgenici αMUPA a lunga vita che sovraesprimono la leptina rispetto ai topi con gestione FVB/N di tipo selvatico (WT).

Per percepire il contributo della leptina al cuore coronarico ischemico, abbiamo misurato proprio qui l’espressione dei geni che codificano per la leptina e le proteine correlate all’ischemia nei topi αMUPA e WT all’interno del cuore coronarico rispetto al tessuto adiposo dopo infarto miocardico. Inoltre, esaminiamo l’espressione genica nei cardiomiociti neonatali di ratto al di sotto dell’ipossia in assenza e presenza di leptina aggiunta esogenamente o di un antagonista della leptina . Abbiamo usato RT-PCR in tempo reale e saggi ELISA o Western blot per misurare, rispettivamente, i range di mRNA e proteine.

I risultati hanno dimostrato che gli intervalli di leptina circolanti e gli intervalli di mRNA di leptina ed eme ossigenasi-1 (HO-1) all’interno del cuore coronarico sono stati elevati in ciascun genotipo di topo dopo 24 ore di infarto miocardico (MI), raggiungendo valori maggiori nei topi αMUPA. Al contrario, l’espressione genica della leptina nel tessuto adiposo era notevolmente elevata solo nei topi WT, raggiungendo tuttavia livelli di riduzione rispetto all’intestino. L’espressione dei geni proinfiammatori che codificano per TNFα, IL-1β è stata inoltre ampiamente elevata dopo MI all’interno del cuore coronarico in ciascuna varietà di topi, raggiungendo comunque intervalli significativamente ridotti nei topi αMUPA che indicano uno stato infiammatorio mitigato.

Nei cardiomiociti, gli intervalli di mRNA di tutti i geni sopra menzionati oltre ai geni HIF-1α e SOD2 sono stati elevati dopo l’ipossia. Il pretrattamento con leptina esogena ha ampiamente abbassato gli intervalli di mRNA di TNFα e IL-1β dopo l’ipossia, migliorando l’espressione di tutti i diversi geni e diminuendo gli intervalli di ROS. Il pretrattamento delle cellule con un antagonista della leptina ha aumentato esclusivamente i gradi di mRNA della leptina , suggerendo una regolazione sfavorevole dell’ormone sull’espressione del suo gene personale.

In totale, i risultati hanno dimostrato che la leptina influisce sull’espressione dei geni nei cardiomiociti al di sotto dell’ipossia in un modo che potrebbe mitigare l’irritazione e lo stress ossidativo, suggerendo lo stesso effetto della leptina endogena nei topi αMUPA. Inoltre, la leptina ha maggiori probabilità di funzionare nel cuore ischemico murino con maggiore successo in un autocrino rispetto al metodo paracrino. Questi risultati suggeriscono che la leptina può ridurre il danno ischemico modulando l’espressione genica all’interno del cuore coronarico.

Prova di una relazione tra leptina plasmatica , non Nesfatin-1, e desiderio nei maschi alcoldipendenti dopo l’astinenza.

Lo scopo di questa ricerca era scoprire se la leptina plasmatica , la nesfatin-1, il cortisolo, il problema neurotrofico di origine cerebrale (BDNF) e le citochine infiammatorie potrebbero essere utilizzati come potenziali biomarcatori per il diploma di craving all’interno del gruppo alcol-dipendente. malati dopo 1 mese di astinenza.

Sono stati valutati un totale di 83 pazienti con disfunzione da uso di alcol (AUD) e 61 argomenti salutari. I malati di AUD sono stati scelti dalla Divisione Dipendenza dai Materiali, Anhui Benessere Psicologico Cuore, e gli argomenti all’interno del gruppo di gestione sono stati scelti da volontari sani.

La scala del questionario sull’urgenza dell’alcol (AUQ) è stata utilizzata per giudicare l’entità del desiderio per l’alcol, e il controllo di screening dell’alcolismo del Michigan (MAST), il controllo di Fagerstrom per la dipendenza da nicotina (FTND), la scala di valutazione del nervosismo (SAS) e la Self-Score Despair Scale (SDS) è stata inoltre valutata in pazienti con AUD. Il saggio ELISA (Enzyme-Linked Immunosorbent Assay ) è stato utilizzato per la misurazione della leptina plasmatica , della nesfatin-1, del cortisolo, del BDNF, dell’interleuchina-6 (IL-6), della proteina C-reattiva (CRP) e del fattore di necrosi tumorale-α (TNF). -α) intervalli. Esaminare con controlli sani, la leptina comune , leptina /BMI, IL-6,Gli intervalli di PCR e TNF-α in chi soffre di AUD sono stati considerevolmente elevati, mentre gli intervalli di BDNF sono stati notevolmente ridotti.

Inoltre, la valutazione della correlazione parziale ha confermato che i punteggi AUQ dei soggetti alcoldipendenti sono stati correlati positivamente con i range di leptina plasmatica ( r = 0,613, P <0,001), relativamente alla nesfatina-1 ( r = 0,066, P = 0,569) dopo aver controllato l’età come covariata. Inoltre, è stato scoperto che gli intervalli plasmatici di nesfatin-1 sono correlati con i punteggi SDS ( r = 0,366, P = 0,001) all’interno del gruppo AUD. Inoltre, gli intervalli di leptina plasmatica sono stati correlati positivamente con IL-6 plasmatico ( r = 0,257, P = 0,033), CRP ( r = 0,305, P = 0,011) e TNF-α ( r = 0,311, P = 0,009) e correlata negativamente agli intervalli BDNF ( r = -0,245, P = 0,042) in chi soffre di AUD.

Questi risultati suggeriscono che la leptina plasmatica , ma non la nesfatin-1, possa essere un possibile biomarker per il diploma di craving in soggetti alcoldipendenti dopo 1 mese di astinenza, il cui meccanismo può essere associato alla disfunzione delle citochine infiammatorie e del BDNF intervalli.

Elevati intervalli di circolazione dell’attivatore del plasminogeno di tipo tissutale sono correlati alla minaccia di aborto spontaneo durante il primo trimestre di gravidanza.

L’aborto spontaneo è una complicanza standard nella gravidanza precoce, con un’incidenza del 20% circa. L’ecografia e la misurazione della gonadotropina corionica umana vengono utilizzate per determinare i malati vulnerabili all’aborto spontaneo; tuttavia, c’è un desiderio scientifico di cercare nuovi biomarcatori per determinare in modo prospettico i malati prima dell’inizio dei segni scientifici.

Proprio qui, ci proponiamo di analizzare potenziali biomarcatori di aborto spontaneo presi all’interno del primo appuntamento scientifico della gravidanza. Una ricerca caso-controllo è stata condotta principalmente sulla base di una coorte raccolta prospetticamente durante la quale i casi e i controlli sono stati stratificati retrospettivamente principalmente in base al risultato finale di gravidanza: gravidanze sane regolari (controlli = 33) e gravidanze che hanno portato ad aborto spontaneo (istanze = 10).

Abbiamo valutato le vescicole extracellulari isolate dalla precipitazione con ExoQuick™ e le concentrazioni proteiche di attivatore tissutale del plasminogeno, leptina e adiponectina misurate mediante ELISA . Le vescicole extracellulari hanno confermato la morfologia standard e le proteine di membrana: CD63, Alix e Flotilin-1. Le distribuzioni in scala delle vescicole extracellulari remote sono state di 112 ± 27 e 118 ± 28 nm di diametro rispettivamente per i controlli e per l’aborto spontaneo e l’intera quantità di vescicole extracellulari non presentava alcuna distinzione tra i controlli e il gruppo di aborto spontaneo.

Acetylcholinesterase Activity Assay Kit - 100 Assays
AR4001-unit	BosterBio	unit	EUR 380.4

MicroMolar ADP Assay Kit - 100 assays
MAD100K	ProFoldin	100 assays	EUR 278.4
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MAD reagent 1, 30 ul of 100 x MAD reagent 2 and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of ADP using a standard black 384-well plate.

MicroMolar GDP assay kit - 100 assays
MGD100K	ProFoldin	100 assays	EUR 278.4
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MGD reagent 1, 30 ul of 100 x MGD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of GDP using a standard black 384-well plate.

MicroMolar UDP assay kit - 100 assays
MUD100K	ProFoldin	100 assays	EUR 278.4
Description: This product includes 350 ul of 10 x Buffer, 30 ul of 100 x MUD reagent 1, 30 ul of 100 x MUD reagent 2, and 300 ul of 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of UDP using a standard black 384-well plate.

Leptin (Human) ELISA Kit
E4653-100	Biovision		EUR 874.8

Leptin (human) ELISA Kit
K4777-100	Biovision	100 assays	EUR 970.8

Description: Sensitive, Colorimetric Assay

Leptin (human) ELISA Kit
K2191-100	ApexBio	100 assays	EUR 1220.4

QuickDetect? Leptin (Human) ELISA Kit
E4449-100	Biovision		EUR 705.6

E. coli BirA - for 100 assays
BIRA-100KEC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli BirA.

E. coli GlmU - for 100 assays
GLMU-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 500 nM E. coli GlmU.

E. coli gyrA - for 100 assays
GYRA-100EC	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 2 uM E.coli gyrA

P. aeruginosa gyrA - for 100 assays
GYRA-100PA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 2 uM P. aeruginosa gyrA

S. aureus gyrA - for 100 assays
GYRA-100SA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 43 ul of 7.5 uM S. aureus gyrA

E. coli gyrB - for 100 assays
GYRB-100EC	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 2 uM E.coli gyrB

P. aeruginosa gyrB - for 100 assays
GYRB-100PA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 2 uM P. aeruginosa gyrB

S. aureus gyrB - for 100 assays
GYRB-100SA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 43 ul of 7.5 uM S. aureus gyrB

E. coli IspD - for 100 assays
ISPD-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 200 nM E. coli IspD.

P. aeruginosa IspD - for 100 assays
ISPD-100PA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 200 nM P. aeruginosa IspD.

E. coli MurA - for 100 assays
MURA-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli MurA.

E. coli MurC - for 100 assays
MURC-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli MurC.

P. aeruginosa MurC - for 100 assays
MURC-100PA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM P. aeruginosa MurC.

S. pneumonia MurC - for 100 assays
MURC-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM S. pneumoniae MurC.

E. coli MurD - for 100 assays
MURD-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 2 uM E. coli MurD.

P. aeruginosa MurD - for 100 assays
MURD-100PA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM P. aeruginosa MurD.

S. aureus MurD - for 100 assays
MURD-100SA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM S. aureus MurD.

E. coli MurE - for 100 assays
MURE-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 5 uM E. coli MurE

E. coli MurF - for 100 assays
MURF-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM E. coli MurF.

P. aeruginosa MurF - for 100 assays
MURF-100PA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM P. aeruginosa MurF.

S. aureus MurF - for 100 assays
MURF-100SA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM S. aureus MurF.

S. pneumonia MurF - for 100 assays
MURF-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM S. pneumoniae MurF.

E. coli parC - for 100 assays
PARC-100EC	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM E. coli parC

P. aeruginosa parC - for 100 assays
PARC-100PA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM P. aeruginosa parC

S. aureus parC - for 100 assays
PARC-100SA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM S. aureus ParC

S. pneumoniae parC - for 100 assays
PARC-100SP	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM S. pneumoniae parC

E. coli parE - for 100 assays
PARE-100EC	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM E. coli parE

P. aeruginosa parE - for 100 assays
PARE-100PA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM P. aeruginosa parE

S. aureus parE - for 100 assays
PARE-100SA	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM S. aureus ParE

S. pneumoniae parE - for 100 assays
PARE-100SP	ProFoldin	100 assays	EUR 246.49
Description: This product includes 50 ul of 5 uM S. pneumoniae parE

S. aureus PncB - for 100 assays
PNCB-100SA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 5 uM S. aureus PNCB.

S. pneumonia PncB - for 100 assays
PNCB-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 5 uM S. pneumoniae PNCB.

Leptin (Mouse) ELISA Kit
E4654-100	Biovision		EUR 874.8

Leptin (Rat) ELISA Kit
E4655-100	Biovision		EUR 874.8

AccuGreen High Sensitivity dsDNA Quantitation Kit (100 assays)
31066-T	Biotium	100kit	EUR 159.6
Description: Minimum order quantity: 1 unit of 100kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (100 assays)
31069-T	Biotium	1kit	EUR 148.8
Description: Minimum order quantity: 1 unit of 1kit

Annexin V, R-phycoerythrin conjugate (100 assays)
9-29045	Biotium	EUR 178.80 EUR 374.40	100uL 500uL
Description: Minimum order quantity: 1 unit of 500uL

Annexin V, Allophycocyanin crosslinked conjugate (100 assays)
9-29057	Biotium	EUR 178.80 EUR 374.40	100uL 500uL
Description: Minimum order quantity: 1 unit of 500uL

E. coli DNA helicase - for 100 assays
DNAB-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 20 uM E. coli DNA helicase.

H. influenza DNA helicase - for 100 assays
DNAB-100HI	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 20 uM H influenzae DNA helicase.

P. aeruginosa DNA helicase - for 100 assays
DNAB-100PA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 20 uM DNA P. aeruginosa helicase.

S. pneumonia DNA helicase - for 100 assays
DNAB-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 20 uM S. pneumoniae DNA helicase.

E. coli DNA primase - for 100 assays
DNAG-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 45 ul of 10 uM E. coli primase-helicase complex

H. influenza DNA primase - for 100 assays
DNAG-100HI	ProFoldin	100 assays	EUR 309.4
Description: This product includes 45 ul of 10 uM H. influenzae primase-helicase complex

S. aureus DNA primase - for 100 assays
DNAG-100SA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 45 ul of 10 uM S. aureus primase.

S. pneumonia DNA primase - for 100 assays
DNAG-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 45 ul of 10 uM S. pneumoniae primase.

E. coli guanylate kinase - for 100 assays
GMK-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul 1 uM E. coli GMP kinase

S. pneumoniae guanylate kinase - for 100 assays
GMK-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul 1 uM S. pneumoniae GMP kinase

E. coli DNA ligase - for 100 assays
LIG-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 10 ul of 500 nM E. coli ligase.

E. coli NAD kinase - for 100 assays
NADK-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 2 uM E. coli UMP kinase

E. coli polynucleotide phosphorylase - for 100 assays
PNP-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 25 uM E. coli PNPase.

E. coli RNA polymerase - for 100 assays
RNAP-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM E.coli RNA Polymerase.

S. aureus RNA polymerase - for 100 assays
RNAP-100SA	ProFoldin	100 assays	EUR 399.28
Description: This product includes 33 ul of 2 uM S. aureus RNA Polymerase.

E. coli DNA gyrase - for 100 assays
TOP2-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes wild-type E. coli gyrase for 100 assays (40 ul of 2000 nM stock solution)

P. aeruginosa DNA gyrase - for 100 assays
TOP2-100PA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 50 ul of 2 uM P. aeruginosa gyrase

S. aureus DNA gyrase - for 100 assays
TOP2-100SA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 43 ul of 7.5 uM S. aureus gyrase.

E. coli UMP kinase - for 100 assays
UMK-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 1uM E. coli UMP kinase

JC-1 Mitochondrial Membrane Potential Detection Kit (100 assays)
30001	Biotium	1KIT	EUR 361.2
Description: Minimum order quantity: 1 unit of 1KIT

SARS-Cov-2 RNA polymerase assay kit-100 (100 assays, enzyme not included)
S2RPA100K	ProFoldin	100 assays	EUR 556
Description: This product includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x NTPs (ATP, UTP, GTP, CTP) and 700 µl of 10 x fluorescence dye. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format. The assay kit includes all reagents except the enzyme.

SARS-Cov-2 RNA polymerase assay kit plus-100 (100 assays, enzyme included)
S2RPA100KE	ProFoldin	100 assays	EUR 1484.8
Description: This product includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x mM NTPs (ATP, UTP, GTP, CTP), 700 µl of 10 x fluorescence dye and 50 µl of 50 x SARS-CoV-2 RdRp. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format.

AccuGreen High Sensitivity dsDNA Quantitation Solution (100 assays)
31068-T	Biotium	100kit	EUR 139.2
Description: Minimum order quantity: 1 unit of 100kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (100 assays)
31070-T	Biotium	1kit	EUR 130.8
Description: Minimum order quantity: 1 unit of 1kit

E. coli DNA polymerase alpha - for 100 assays
DPA-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM E. coli DNA polymerase III alpha.

H. influenza DNA polymerase alpha - for 100 assays
DPA-100HI	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM H. influenzae DNA polymerase III alpha.

S. pneumonia DNA polymerase alpha - for 100 assays
DPA-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 2 uM S. pneumoniae DNA polymerase III alpha.

E. coli thymidylate kinase (TMK) - for 100 assays
TMK-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 35 ul of 1uM E. coli Thymidylate kinase

E. coli DNA topoisomerase I - for 100 assays
TOP1-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 42 ul of 4 uM E. coli topoisomerase I

E. coli topoisomerase IV holoenzyme - for 100 assays
TOP4-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 50 ul of 5 uM E. coli topoisomerase IV (parC-parE)

P. aeruginosa DNA topoisomerase IV - for 100 assays
TOP4-100PA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 50 ul of 5 uMP. aeruginosa topoisomerase IV (parC-parE)

S. aureus DNA topoisomerase IV - for 100 assays
TOP4-100SA	ProFoldin	100 assays	EUR 309.4
Description: This product includes 50 ul of 5 uM S. aureus DNA topoisomerase IV.

S. pneumoniae DNA topoisomerase IV - for 100 assays
TOP4-100SP	ProFoldin	100 assays	EUR 309.4
Description: This product includes 50 ul of 5 uM S. pneumoniae DNA topoisomerase IV

Human Leptin R(Leptin receptor) ELISA Kit
EH0217	FN Test	96T	EUR 628.92

Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

NucView 488 and MitoView 633 Apoptosis Assay Kit (100 assays)
30062	Biotium	1KIT	EUR 514.8
Description: Minimum order quantity: 1 unit of 1KIT

NucView 488 and RedDot 2 Apoptosis & Necrosis Kit (100 assays)
30072	Biotium	1KIT	EUR 458.4
Description: Minimum order quantity: 1 unit of 1KIT

Human Leptin ELISA kit
55R-1635	Fitzgerald	1 kit	EUR 571.2
Description: ELISA kit for the detection of Human Leptin in the research laboratory

Human Leptin ELISA kit
55R-1998	Fitzgerald	96 tests	EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA kit
55R-2265	Fitzgerald	96 tests	EUR 1099.2
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA Kit
DEIA1165	Creative Diagnostics	96T	EUR 878.4
Description: The Human LEP ELISA kit is designed to detect and quantify the level of Human LEP in cell culture supernatant, serum, plasma and tissue. This kit is for research use only and not intended for diagnostic purposes.

Human Leptin ELISA Kit
E-80LPT	Immunology Consultants Laboratory	1 x 96 well plate	EUR 486

Human Leptin ELISA kit
E01L0038-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit
E01L0038-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit
E01L0038-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA Kit
EHL0045	Abclonal	96Tests	EUR 625.2

Human Leptin ELISA kit
LF-EK50217	Abfrontier	1Ã—96T	EUR 777.6

Human Leptin ELISA Kit
RK00035	Abclonal	96 Tests	EUR 625.2

Gel-based E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus-100 (for 100 assays)
GDSA100KE	ProFoldin	100 assays	EUR 399.28
Description: This product includes 400 ul of 10 x Buffer, 105 ul of 250 ug/ml relaxed DNA, 220 ul of 10 mM ATP, 550 ul of 5 x Gel loading buffer and 25 ul of 2000 nM E.coli gyrase.

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
ELISA-1	Alpha Diagnostics	1	EUR 242.4

Leptin Antibody
5366-100	Biovision		EUR 379.2

Leptin Antibody
5367-100	Biovision		EUR 379.2

Leptin Antibody
5368-100	Biovision		EUR 379.2

Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)
30008-2	Biotium	1KIT	EUR 459.6
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)
30011-2	Biotium	1KIT	EUR 459.6
Description: Minimum order quantity: 1 unit of 1KIT

Human Leptin ELISA Kit, 96 tests, Quantitative, 96 tests
100-135-LEH	Alpha Diagnostics	1 kit	EUR 781.2

Human Leptin (LEP) ELISA Kit
20-abx152192	Abbexa	EUR 6448.80 EUR 3441.60 EUR 811.20	10 × 96 tests 5 × 96 tests 96 tests

Human Leptin (LEP) ELISA Kit
abx050142-96tests	Abbexa	96 tests	EUR 801.6

Human Leptin (LEP) ELISA Kit
abx051330-96tests	Abbexa	96 tests	EUR 801.6

Human Leptin (LEP) ELISA Kit
abx251497-96tests	Abbexa	96 tests	EUR 801.6

Human Leptin (LEP) ELISA Kit
abx570296-96tests	Abbexa	96 tests	EUR 801.6

Human Leptin,LEP ELISA kit
201-12-1560	SunredBio	96 tests	EUR 528

Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Leptin, LEP ELISA kit
CSB-E04649h-24T	Cusabio	1 plate of 24 wells	EUR 198

Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Leptin, LEP ELISA kit
1-CSB-E04649h	Cusabio	EUR 600.00 EUR 4082.40 EUR 2184.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each

Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Leptin, LEP ELISA Kit
DEIA167	Creative Diagnostics	96T	EUR 888
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitative measurement of Human leptin in serum, plasma and other biological fluids.

Human Leptin,LEP ELISA kit
CN-03239H1	ChemNorm	96T	EUR 530.4

Human Leptin,LEP ELISA kit
CN-03239H2	ChemNorm	48T	EUR 351.6

Human Leptin (LEP) ELISA Kit
DLR-LEP-Hu-48T	DL Develop	48T	EUR 470.4

Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin (LEP) ELISA Kit
DLR-LEP-Hu-96T	DL Develop	96T	EUR 602.4

Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin Antibody ELISA kit
E01L0039-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Antibody ELISA kit
E01L0039-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin Antibody ELISA kit
E01L0039-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Human Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit
E01L0273-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit
E01L0273-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin receptor ELISA kit
E01L0273-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Human Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Leptin ELISA Kit (Human) (OKBB00206)
OKBB00206	Aviva Systems Biology	96 Tests	EUR 606
Description: Description of target: Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 10 pg/mL

Human LEP(Leptin) ELISA Kit
EH0216	FN Test	96T	EUR 571.5

Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human Leptin PicoKine ELISA Kit
EK0437	BosterBio	96 wells	EUR 510
Description: For quantitative detection of human Leptin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Leptin R ELISA KIT\|Human
EF007100	Lifescience Market	96 Tests	EUR 826.8

Human LEP/ Leptin ELISA Kit
E1447Hu	Sunlong	1 Kit	EUR 644.4

Human Leptin ELISA kit (4X96T)
LF-EK50218	Abfrontier	4Ã—96T	EUR 2641.2

ELISA kit for Human Leptin
KET6026-48T	Abbkine	48T	EUR 242.4

Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin
KET6026-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 1292.4

Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin
KET6026-96T	Abbkine	96T	EUR 346.8

Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Leptin(LEP)ELISA Kit
GA-E1576HM-48T	GenAsia Biotech	48T	EUR 346.8

Human Leptin(LEP)ELISA Kit
GA-E1576HM-96T	GenAsia Biotech	96T	EUR 559.2

Human Leptin AssayMax ELISA Kit
EL2001-1	AssayPro	96 Well Plate	EUR 500.4

Human Leptin Receptor ELISA Kit
ELA-E0961h	Lifescience Market	96 Tests	EUR 988.8

Human Leptin (LEP) ELISA Kit
SEA084Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 3855.67

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
SEA084Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 422.7

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
SEA084Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 552.43

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
SEA084Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2125.94

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
4-SEA084Hu	Cloud-Clone	EUR 3916.80 EUR 2066.40 EUR 553.20	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Leptin (LEP) ELISA Kit
RDR-LEP-Hu-48Tests	Reddot Biotech	48 Tests	EUR 475.2

Human Leptin (LEP) ELISA Kit
RDR-LEP-Hu-96Tests	Reddot Biotech	96 Tests	EUR 654

Human Leptin(LEP)ELISA Kit
QY-E01495	Qayee Biotechnology	96T	EUR 433.2

Human Leptin (LEP) ELISA Kit
RD-LEP-Hu-48Tests	Reddot Biotech	48 Tests	EUR 456

Human Leptin (LEP) ELISA Kit
RD-LEP-Hu-96Tests	Reddot Biotech	96 Tests	EUR 625.2

Leptin R ELISA Kit\| Mouse Leptin Receptor ELISA Kit
EF012890	Lifescience Market	96 Tests	EUR 826.8

Mouse Leptin ELISA Kit, 96 tests, Quantitative
100-130-LEM	Alpha Diagnostics	1 Kit	EUR 854.4

Mouse Leptin R(Leptin Receptor) ELISA Kit
EM0130	FN Test	96T	EUR 628.92

Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Recombinant Human Leptin Triple Antagonist
CG072-100	GenDepot	100ug	EUR 489.6

ViaFluor 488 SE Cell Proliferation Kit, Trial Size (1 vial, 100 assays)
30086-T	Biotium	1KIT	EUR 124.8
Description: Minimum order quantity: 1 unit of 1KIT

E. coli D-Ala-D-Ala ligase - for 100 assays
DDL-100EC	ProFoldin	100 assays	EUR 309.4
Description: This product includes 33 ul of 200 nM E. coli D-Ala:D-Ala ligase.

Leptin Receptor Antibody
5582-100	Biovision		EUR 405.6

Human Soluble protein-100,S-100 ELISA Kit
201-12-1290	SunredBio	96 tests	EUR 528

Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Soluble protein-100, S-100 ELISA Kit
CSB-E08067h-24T	Cusabio	1 plate of 24 wells	EUR 198

Description: Quantitativesandwich ELISA kit for measuring Human Soluble protein-100, S-100 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Soluble protein-100, S-100 ELISA Kit
1-CSB-E08067h	Cusabio	EUR 843.60 EUR 5811.60 EUR 3084.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each

Description: Quantitativesandwich ELISA kit for measuring Human Soluble protein-100, S-100 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Soluble protein-100,S-100 ELISA Kit
CN-03332H1	ChemNorm	96T	EUR 538.8

Human Soluble protein-100,S-100 ELISA Kit
CN-03332H2	ChemNorm	48T	EUR 358.8

Human Soluble protein-100(S-100)ELISA Kit
GA-E1306HM-48T	GenAsia Biotech	48T	EUR 346.8

Human Soluble protein-100(S-100)ELISA Kit
GA-E1306HM-96T	GenAsia Biotech	96T	EUR 559.2

Human Soluble protein- 100, S- 100 ELISA Kit
SELI-66017h	Lifescience Market	96 Tests	EUR 788.4

Human S-100(Soluble Protein-100) ELISA Kit
STJ150540	St John's Laboratory	1 kit	EUR 494.4
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S-100 in human serum, plasma and other biological fluids

Human Soluble protein-100(S-100)ELISA Kit
QY-E04630	Qayee Biotechnology	96T	EUR 433.2

Mouse Leptin ELISA Kit
55R-1636	Fitzgerald	1 kit	EUR 660
Description: ELISA kit for detection of Leptin in the research laboratory

Rat Leptin ELISA kit
55R-1953	Fitzgerald	96 tests	EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Bovine Leptin ELISA kit
55R-1959	Fitzgerald	96 tests	EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Rabbit Leptin ELISA kit
55R-2006	Fitzgerald	96 tests	EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Mouse Leptin ELISA kit
55R-2027	Fitzgerald	96 tests	EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Porcine Leptin ELISA kit
55R-2032	Fitzgerald	96 tests	EUR 740.4
Description: ELISA Kit for detection of Leptin in the research laboratory

Mouse Leptin ELISA Kit
E-90LPT	Immunology Consultants Laboratory	1 x 96 well plate	EUR 529.2

Goat Leptin ELISA kit
E06L0038-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Leptin ELISA kit
E06L0038-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Leptin ELISA kit
E06L0038-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit
E04L0038-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit
E04L0038-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit
E04L0038-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit
E03L0038-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit
E03L0038-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA kit
E03L0038-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

L’attivatore tissutale del plasminogeno ha confermato una grande distinzione (p = 0,0004) tra ciascuna squadra, sebbene né l’adiponectina né la leptina abbiano rivelato importanti aggiustamenti, indicando che le ragazze che hanno avuto aborti spontanei hanno gamme considerevolmente maggiori di attivatore tissutale del plasminogeno rispetto alle donne che hanno avuto gravidanze regolari.