Livello di adiponectina, leptina e metalloproteinasi della matrice selezionate in pazienti in sovrappeso di sesso femminile con gonartrosi primaria

Level of Adiponectin, Leptin and Selected Matrix Metalloproteinases in Female Overweight Patients with Primary Gonarthrosis
L’obiettivo di questa ricerca era valutare i range di adiponectina (ACRP-30) e  leptina  nel siero e nel liquido sinoviale (SF) di donne obese con gonartrosi maggiore (GOA) e ricercare le loro correlazioni con la posizione scientifica e l’entità dell’OA biochimica biomarcatori: metalloproteinasi di matrice (MMP) 1, MMP-9 e inibitore tissutale della metalloproteinasi (TIMP-1). Il gruppo studiato era composto da 39 donne obese che hanno subito un intervento di artroplastica completa del ginocchio a causa di GOA.
Lo stadio di GOA è stato valutato in risposta ai punteggi Knee Society Rating (KSS), Ahlbäck e Kellgren-Lawrence (Ok-L). Erano stati prelevati sangue periferico e SF. Gli intervalli di biomarcatori scelti sono stati valutati utilizzando   kit ELISA . Lo stadio implicato di ACRP-30 nel siero valutato era di 8393,80 ng/mL e in SF, 774,33 ng/mL, il focus principale di  leptina  nel siero era 32.040,74 pg/mL e in SF, 27.332,12 pg/mL. Intervalli di  leptina  nel siero e SF correlati con l’indice di massa corporea (BMI), ( p  = 0,0005 e  p  = 0,0002, rispettivamente).
Gli intervalli di ACRP-30 nel siero sono stati correlati con i punteggi scientifici (Ahlbäck:  p  = 0,0214; Ok-L:  p  = 0,0146). ACRP-30 in SF correlato con ACRP-30 nel siero ( p  = 0,0003), tendeva a correlare negativamente con MMP-1 nel siero ( p  = 0,0598) e correlare positivamente con pro-MMP-1 in SF ( p  = 0,0600). Per concludere, questa ricerca conferma le correlazioni tra le concentrazioni di ciascuno,  leptina  e ACRP-30, valutando siero e SF. Nelle femmine obese, i   livelli di leptina migliorano con l’IMC e lo stadio sierico ACRP-30 migliora con ulteriori livelli superiori di GOA. Infine, la  leptina gli intervalli erano stati correlati con il focus sierico di TIMP-1, uno dei tanti marcatori biochimici di GOA.

Le cellule adipose pancreatiche delle persone con diabete di tipo 2 mostrano un ridotto esercizio adipogenico e lipolitico

I problemi di peso, in particolare l’accumulo di grassi viscerali, aumenteranno la possibilità di diabete di tipo 2 (T2D). Lo scopo di questa ricerca era di studiare l’influenza del diabete di tipo 2 sul deposito di grassi pancreatici. Sono stati raccolti cuscinetti di grasso pancreatico da 17 pazienti parzialmente pancreatectomizzati (PPP), preadipociti pancreatici isolati e differenziati in vitro. I malati sono stati raggruppati utilizzando HbA1c in normali tolleranti al glucosio (NGT), prediabetici (PD) e T2D. I profili del trascrittoma di preadipociti e adipociti sono stati valutati mediante RNAseq.
La sensibilità all’insulina è stata stimata quantificando la fosforilazione di AKT su western blot. La capacità lipogenica è stata valutata con Otaining rosa olio, esercizio lipolitico attraverso il lancio di acidi grassi. Gli elementi secreti sono stati misurati utilizzando  ELISA . La valutazione comparativa del trascrittoma di preadipociti e adipociti indica una sovraregolazione difettosa dei geni che governano l’adipogenesi (NR1H3), la lipogenesi (FASN, SCD, ELOVL6, FADS1) e la lipolisi (LIPE) durante la differenziazione delle cellule da T2D-PPP.
Inoltre, il rapporto tra  leptina /adiponectina mRNA era maggiore in T2D che in NGT-PPP. I preadipociti e gli adipociti di NGT-PPP erano più sensibili all’insulina rispetto alle cellule T2D-PPP per quanto riguarda la fosforilazione di AKT. L’accumulo di trigliceridi era paragonabile negli adipociti NGT e T2D. Indipendentemente da un’eccessiva espressione dei recettori NPR1 e NPR2 negli adipociti NGT e T2D, la lipolisi è stata stimolata da ANP 1,74 volte esclusivamente nelle cellule NGT.
Questa stimolazione è stata ulteriormente elevata dal dipiridamolo inibitore della PDE5 (3,09 volte). Il dipiridamolo e la forskolina hanno aumentato il recettore della lipolisi in modo indipendente di 1,88 volte e 1,48 volte, rispettivamente, esclusivamente nelle cellule NGT. In conclusione, lo stato metabolico influenza in modo persistente la differenziazione e la lipolisi degli adipociti pancreatici. Queste alterazioni potrebbero peggiorare l’evento di T2D.

Esercizio antiproliferativo dell’agonista del recettore del peptide-1 simile al glucagone sulla mammella correlata ai problemi di peso La maggior parte dei tumori: l’influenza sulla modulazione dell’espressione delle adipochine negli adipociti e nella maggior parte delle cellule tumorali

I problemi di peso sono legati al pericolo eccessivo e alla prognosi infausta della maggior parte dei tumori al seno (BC). I problemi di peso favoriscono la proliferazione delle cellule BC modulando la produzione di adipochine, insieme ad adiponectina (anti-neoplasticadipochina), leptina  (adipochina cancerogena) e mediatori dell’infiammazione.
Nell’ambito della ricerca attuale abbiamo studiato i risultati anti-proliferativi di liraglutide (LG; farmaco antidiabetico e per la riduzione del peso) su cellule BC umane MCF-7 coltivate in terreno condizionato da cellule staminali derivate da tessuto adiposo in sovrappeso (ADSCs-CM) e se o meno questo impatto è mediato dalla modulazione delle adipochine nelle ADSC e nella maggior parte delle cellule tumorali. La proliferazione è stata studiata utilizzando il controllo di vitalità AlamarBlue, il test di formazione di colonie e la valutazione del ciclo cellulare.
Gli intervalli e l’espressione delle adipochine e dei loro recettori sono stati saggiati utilizzando  ELISA  e RT-PCR. LG ha provocato l’inibizione del 48% della proliferazione MCF-7 in ADSCs-CM in sovrappeso, ha ridotto la formazione di colonie e ha indotto l’arresto della parte G0/G1. LG ha inoltre ridotto i gradi dei mediatori dell’infiammazione, soppresso l’espressione della  leptina , mentre elevati intervalli di mRNA di adiponectina e dei loro recettori nelle ADSC in sovrappeso e nella maggior parte delle cellule tumorali coltivate in ADCS-CM in sovrappeso.
In conclusione, LG potrebbe mitigare lo sviluppo delle cellule BC in argomenti in sovrappeso; per questo motivo può benissimo essere utilizzato per la prevenzione scientifica e/o il rimedio del BC in soggetti in sovrappeso. Potrebbe aiutare a migliorare i risultati del trattamento e ridurre il tasso di mortalità nei pazienti in sovrappeso con BC.
Livello di adiponectina, leptina e metalloproteinasi della matrice selezionate in pazienti in sovrappeso di sesso femminile con gonartrosi primaria

Aptasensor per la quantificazione della  leptina  tramite PCR Amplificazione di brevi DNA-aptameri

La quantificazione delle proteine ​​è storicamente effettuata mediante test di immunoassorbimento enzimatico ( ELISA ), che comporta lunghi tempi di preparazione. Per battere questo, i nuovi approcci utilizzano gli aptameri come scelta alternativa agli anticorpi. In questo documento, presentiamo un metodo nuovo di zecca per quantificare le proteine ​​con brevi aptameri di DNA mediante risposta a catena della polimerasi (PCR) che porta a istanze di protocollo più brevi con limiti di rilevamento relativamente migliorati.
La metodologia proposta presenta una nuova opzione per quantificare contemporaneamente sia la proteina obiettivo che i corrispondenti brevi aptameri di DNA, che ci consente inoltre di caratterizzare completamente l’efficienza degli aptasensori. La leptina umana   viene utilizzata come proteina obiettivo per convalidare questo sistema, poiché è considerato un biomarcatore vitale per la ricerca sull’obesità.
Nei nostri esperimenti, abbiamo raggiunto il limite inferiore di rilevamento di 100 pg/mL in meno di 2 ore, un limite influenzato dalla dissociazione fissata  dall’aptamero  leptinico, che può benissimo essere migliorato scegliendo un aptamero in più. Grazie al metodo semplice ed economico, questo sistema potrebbe essere impiegato per implementazioni Lab-On-Chip e per una rapida quantificazione “in loco” delle proteine.

La leptina  stimola Akt nella maggior parte delle cellule tumorali esofagee attraverso una serie di piccole GTPasi sensibili all’atorvastatina

I problemi di peso sono un problema pericoloso per l’esofago di Barrett e l’adenocarcinoma esofageo. Il tessuto adiposo secerne l’ormone  leptina . La leptina  è un problema di sviluppo per un certo numero di tipi di cellule, insieme alle cellule di Barrett e alle cellule di adenocarcinoma esofageo. Le statine sono correlate alla riduzione delle cariche dell’esofago di Barrett e della maggior parte dei tumori esofagei e mostrano risultati antitumorali in vitro. I meccanismi di tali risultati non saranno del tutto stabiliti.
Abbiamo ora esaminato le conseguenze della  leptina  e della statina liposolubile, atorvastatina, sulla segnalazione tramite proteine ​​​​leganti GTP monomeriche e Akt. La proliferazione e l’apoptosi sono state valutate nelle cellule OE33. L’esercizio di Akt è stato quantificato mediante ELISA cellulare   e test della chinasi in vitro. Particolari inibitori di piccole molecole e un assemblaggio dominante-negativo erano stati usati per ridurre l’esercizio di Akt. Piccole GTPasi erano state inibite utilizzando la trasfezione di plasmidi dominanti negativi, inibitori della prenilazione e pretrattamento con atorvastatina.
La leptina ha  stimolato l’esercizio di Akt e la proliferazione cellulare e ha inibito l’apoptosi indotta dalla camptotecina in un metodo Akt-sensibile. La leptina ha  indotto la fosforilazione di Dangerous e FOXO1 in un metodo Akt-sensibile. La leptina ha  attivato Ras, Rac, RhoA e cdc42. La trasfezione di plasmidi dominanti-negativi ha confermato che l’  attivazione di Akt indotta dalla leptina richiedeva Ras, RhoA cdc42 ma non Rac. L’atorvastatina ha inibito l’  attivazione indotta dalla leptina di Ras, RhoA, cdc42 e Akt. Il co-trattamento con mevalonato ha impedito questi risultati dell’atorvastatina.

Mouse Leptin ELISA Kit

EKF57769-5x96T 5x96T
EUR 2693.25

Mouse Leptin ELISA Kit

EKF57769-96T 96T
EUR 567

Mouse leptin ELISA KIT

E42EM-052 96T/48T Ask for price

Mouse Leptin ELISA Kit

E28L0313 96T
EUR 666.67

Mouse Leptin ELISA Kit

EK1237 96 tests
EUR 511

Mouse Leptin ELISA Kit

55R-1636 1 kit
EUR 615
Description: ELISA kit for detection of Leptin in the research laboratory

Mouse Leptin ELISA kit

55R-2027 96 tests
EUR 658
Description: ELISA Kit for detection of Leptin in the research laboratory

Mouse Leptin ELISA Kit

EM0129 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 37.5pg/ml

Mouse Leptin ELISA Kit

GWB-SKR091 96 Tests Ask for price

Mouse Leptin ELISA Kit

EML0045 96Tests
EUR 625.2

Mouse Leptin ELISA Kit

90030 96
EUR 400

Mouse Leptin R(Leptin Receptor) ELISA Kit

EM0130 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 18.75pg/ml

Leptin ELISA Kit| Mouse

EF012889 96 Tests
EUR 826.8

Mouse Leptin ELISA Kit(LEP)

RK00380 96 Tests
EUR 280

Mouse Lep/ Leptin ELISA Kit

E0859Mo 1 Kit
EUR 543
Description: Ob,Obesity factor

Mouse LEP (Leptin) ELISA Kit

EKE61488-5x96T 5x96T
EUR 2667.6

Mouse LEP (Leptin) ELISA Kit

EKE61488-96T 96T
EUR 561.6

Mouse LEP(Leptin) ELISA Kit

ELK1161-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse LEP(Leptin) ELISA Kit

ELK1161-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse Lep / Leptin ELISA Kit

E0084m 96T
EUR 776

Leptin (Mouse) ELISA Kit

E4654-100 each
EUR 874.8

Nori® Mouse Leptin ELISA Kit

GR117003 96-well
EUR 461

Mouse Leptin (LEP) ELISA Kit

abx050143-96tests 96 tests
EUR 801.6

Mouse Leptin (LEP) ELISA Kit

abx051335-96tests 96 tests
EUR 904.8

Mouse Leptin (LEP) ELISA Kit

abx254349-96tests 96 tests
EUR 877.2

Mouse Leptin (LEP) ELISA Kit

abx570297-96tests 96 tests
EUR 877.2

Mouse Leptin (LEP) ELISA Kit

20-abx154317
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Leptin (LEP) ELISA Kit

DLR-LEP-Mu 96T
EUR 432
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin (LEP) ELISA Kit

DLR-LEP-Mu-48T 48T
EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin (LEP) ELISA Kit

DLR-LEP-Mu-96T 96T
EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin (LEP) ELISA Kit

EKL54769-5x96T 5x96T
EUR 3367.75

Mouse Leptin (LEP) ELISA Kit

EKL54769-96T 96T
EUR 709

Mouse leptin (LEP) Elisa Kit.

EK730391 96 Wells
EUR 0.68

Mouse Leptin,LEP ELISA kit

EKC37269-48T 48T
EUR 328.86

Mouse Leptin,LEP ELISA kit

EKC37269-5x96T 5x96T
EUR 2231.55

Mouse Leptin,LEP ELISA kit

EKC37269-96T 96T
EUR 469.8

Mouse Leptin (LEP) ELISA Kit

EKN46705-48T 48T
EUR 253.26

Mouse Leptin (LEP) ELISA Kit

EKN46705-5x96T 5x96T
EUR 1718.55

Mouse Leptin (LEP) ELISA Kit

EKN46705-96T 96T
EUR 361.8

Mouse Leptin, LEP ELISA Kit

ELA-E0084m 96 Tests
EUR 1038

Mouse Leptin (LEP) ELISA Kit

EKU11213-48T 48T
EUR 446.04

Mouse Leptin (LEP) ELISA Kit

EKU11213-5x96T 5x96T
EUR 3026.7

Mouse Leptin (LEP) ELISA Kit

EKU11213-96T 96T
EUR 637.2

Mouse Leptin (LEP) ELISA Kit

DL-LEP-Mu 96T
EUR 412
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin, Lep ELISA Kit

DEIA130 10 plates
EUR 2994
Description: Mouse Leptin ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant Leptin in a sandwich ELISA format within the range of 20-1,250 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay Leptin in approximately 1,000 ELISA plate wells.

Mouse Leptin (LEP) ELISA Kit

AE35758MO-48Tests 48 Tests
EUR 200
Description: Mouse (Mus musculus)

Mouse Leptin (LEP) ELISA Kit

AE35758MO-96Tests 96 Tests
EUR 360
Description: Mouse (Mus musculus)

Mouse Leptin, LEP ELISA kit

CSB-E04650m-24T 1 plate of 24 wells
EUR 198
Description: Quantitative sandwich ELISA kit for measuring Mouse Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Leptin, LEP ELISA kit

1-CSB-E04650m
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Mouse Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Leptin (LEP) ELISA Kit

EKN52324-48T 48T
EUR 277.55

Mouse Leptin (LEP) ELISA Kit

EKN52324-5x96T 5x96T
EUR 1883.38

Mouse Leptin (LEP) ELISA Kit

EKN52324-96T 96T
EUR 396.5

Mouse Leptin (LEP) ELISA Kit

EKU05594-48T 48T
EUR 371.21

Mouse Leptin (LEP) ELISA Kit

EKU05594-5x96T 5x96T
EUR 2518.93

Mouse Leptin (LEP) ELISA Kit

EKU05594-96T 96T
EUR 530.3

Mouse Leptin (LEP) ELISA Kit

KTE71186-48T 48T
EUR 359
Description: Mouse Leptin (LEP) ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples.

Mouse Leptin (LEP) ELISA Kit

KTE71186-96T 96T
EUR 619
Description: Mouse Leptin (LEP) ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples.

Mouse Leptin (LEP) ELISA Kit

KTE71186-96T5 96 T×5
EUR 2809
Description: Mouse Leptin (LEP) ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples.

Mouse Leptin (LEP) ELISA Kit

KTE71186-96T50 96 T×50
EUR 26209
Description: Mouse Leptin (LEP) ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples.

Mouse Leptin(LEP)ELISA Kit        

GA-E0665MS-48T 48T
EUR 403.2

Mouse Leptin(LEP)ELISA Kit        

GA-E0665MS-96T 96T
EUR 640.8

Mouse Leptin(Lep)ELISA Kit

NSL1434m 96 Test
EUR 528

Mouse Leptin (LEP) ELISA Kit

RD-LEP-Mu-48T 48T
EUR 422.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

RD-LEP-Mu-48Tests 48 Tests
EUR 586.8

Mouse Leptin (LEP) ELISA Kit

RD-LEP-Mu-96T 96T
EUR 603.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

RD-LEP-Mu-96Tests 96 Tests
EUR 812.4

Mouse Leptin (LEP) ELISA Kit

RDR-LEP-Mu-48T 48T
EUR 443.42
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

RDR-LEP-Mu-48Tests 48 Tests
EUR 613.2

Mouse Leptin (LEP) ELISA Kit

RDR-LEP-Mu-96T 96T
EUR 633.47
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

RDR-LEP-Mu-96Tests 96 Tests
EUR 850.8

Mouse leptin (LEP) ELISA Kit.

SL0608Mo -
EUR 528

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-10x96wellstestplate 10x96-wells test plate
EUR 3855.67
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-1x48wellstestplate 1x48-wells test plate
EUR 422.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-1x96wellstestplate 1x96-wells test plate
EUR 552.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-5x96wellstestplate 5x96-wells test plate
EUR 2125.94
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

4-SEA084Mu
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  • Ask for price
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Leptin,LEP ELISA kit        

YLA0175MO-48T 48T Ask for price

Mouse Leptin,LEP ELISA kit        

YLA0175MO-96T 96T Ask for price

Leptin ELISA, Mouse

KT-380 1 kit
EUR 931

Nori® Mouse Leptin R ELISA Kit

GR117437 96-well
EUR 461

Leptin R ELISA Kit (Mouse) (OKBB00209)

OKBB00209 96 Tests
EUR 621.6
Description: Description of target: Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 5 pg/mL

Mouse Leptin Antibody ELISA kit

E01A23060 96T
EUR 700
Description: ELISA

Mouse Leptin receptor ELISA kit

E01A23121 96T
EUR 700
Description: ELISA

Mouse Leptin Antibody ELISA kit

E03L0039-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin Antibody ELISA kit

E03L0039-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin Antibody ELISA kit

E03L0039-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor ELISA kit

E03L0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor ELISA kit

E03L0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor ELISA kit

E03L0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor ELISA Kit

EK1229 96 tests
EUR 599

Mouse Leptin Receptor ELISA kit

55R-1638 1 kit
EUR 633
Description: ELISA kit for the detection of Leptin Receptor in the research laboratory

Mouse Leptin Receptor ELISA Kit

IMSLEPRKT each
EUR 713
Description: Mouse Leptin Receptor ELISA Kit

Mouse Leptin receptor ELISA Kit

LF-EK50834 1×96T
EUR 777.6

OKAG00091-96W - Leptin ELISA Kit (Mouse)

OKAG00091-96W 1plate:12x8-WellMicrostrips
EUR 475

OKRC01238-96W - Leptin ELISA Kit (Mouse)

OKRC01238-96W 96Wells
EUR 525

ELISA kit for Mouse Leptin (LEP)

KTE71186-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Mouse Leptin (LEP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse LEP (Leptin)

E-EL-M0039 1 plate of 96 wells
EUR 452.4
Description: A sandwich ELISA kit for quantitative measurement of Mouse LEP (Leptin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse LEP (Leptin)

ELK1161 1 plate of 96 wells
EUR 446.4
Description: A sandwich ELISA kit for detection of Leptin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Camel Leptin(Leptin) Elisa Kit

EK765057 96 Wells
EUR 0.96

Human Leptin(Leptin) Elisa Kit

EK710057 96 Wells
EUR 0.56

Mouse Leptin AssayMax™ ELISA Kit

EML2001-1 96 Well Plate
EUR 425

Mouse Leptin Receptor (LEPR) ELISA Kit

abx254357-96tests 96 tests
EUR 848.4

Mouse Leptin Receptor (LEPR) ELISA Kit

abx575468-96tests 96 tests
EUR 848.4

Mouse Leptin Receptor (LEPR) ELISA Kit

20-abx154318
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Mu 96T
EUR 432
Description: serum, plasma, tissue homogenates or other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Mu-48T 48T
EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin Receptor (LEPR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Mu-96T 96T
EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin Receptor (LEPR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Leptin receptor(LEPR) ELISA kit

E01A23164 96T
EUR 700
Description: ELISA

Mouse Leptin receptor(LEPR) ELISA kit

E03L0327-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor(LEPR) ELISA kit

E03L0327-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor(LEPR) ELISA kit

E03L0327-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin Receptor (LEPR) ELISA Kit

EKN46710-48T 48T
EUR 350.42

Mouse Leptin Receptor (LEPR) ELISA Kit

EKN46710-5x96T 5x96T
EUR 2377.85

Mouse Leptin Receptor (LEPR) ELISA Kit

EKN46710-96T 96T
EUR 500.6

Mouse Leptin Receptor (LEPR) ELISA Kit

DL-LEPR-Mu 96T
EUR 412
Description: serum, plasma, tissue homogenates or other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

EKU05599-48T 48T
EUR 514.08

Mouse Leptin Receptor (LEPR) ELISA Kit

EKU05599-5x96T 5x96T
EUR 3488.4

Mouse Leptin Receptor (LEPR) ELISA Kit

EKU05599-96T 96T
EUR 734.4

Mouse Leptin Receptor(LEPR)ELISA Kit

QY-E20899 96T
EUR 433.2

Mouse Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Mu-48T 48T
EUR 422.3
Description: serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Mu-48Tests 48 Tests
EUR 586.8

Mouse Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Mu-96T 96T
EUR 603.3
Description: serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Mu-96Tests 96 Tests
EUR 812.4

Mouse Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Mu-48T 48T
EUR 443.42
Description: serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Mu-48Tests 48 Tests
EUR 613.2

Mouse Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Mu-96T 96T
EUR 633.47
Description: serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Mu-96Tests 96 Tests
EUR 850.8

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-10x96wellstestplate 10x96-wells test plate
EUR 5269.39
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-1x48wellstestplate 1x48-wells test plate
EUR 539.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-1x96wellstestplate 1x96-wells test plate
EUR 718.75
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-5x96wellstestplate 5x96-wells test plate
EUR 2874.38
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

4-SEA083Mu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Leptin Receptor (LEPR) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Mouse Leptin ELISA Kit, 96 tests, Quantitative

100-130-LEM 1 Kit
EUR 854.4

Mouse Lepr/ Leptin receptor ELISA Kit

E0860Mo 1 Kit
EUR 543
Description: Db,Obr,B219,OB receptor,OB-R,CD295

Mouse LEPR(Leptin Receptor) ELISA Kit

ELK2282-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEPR in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse LEPR(Leptin Receptor) ELISA Kit

ELK2282-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEPR in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse Lepr / Leptin receptor ELISA Kit

E0083m 96T
EUR 776

Leptin ELISA Kit (Mouse) : 96 Wells (OKAG00091)

OKAG00091 96 Wells
EUR 715.2
Description: Description of target: Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed:15899045, PubMed:16825198, PubMed:11373681, PubMed:12594516, PubMed:20620997). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) (PubMed:8589726, PubMed:10660043, PubMed:25383904, PubMed:25060689, PubMed:9732873, PubMed:12594516). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity) (PubMed:20620997, PubMed:11373681). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity) (PubMed:16825198, PubMed:20620997). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (By similarity). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed:16825198). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (Probable). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (PubMed:15899045). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4+CD25- T cells proliferation and reduces autophagy during TCR (T cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (PubMed:25060689).;Species reactivity: Mouse;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 20 pg/mL

OKRC01239-96W - Leptin R ELISA Kit (Mouse)

OKRC01239-96W 96Wells
EUR 525

Mouse Leptin receptor PicoKine ELISA Kit

EK0440 96 wells
EUR 523.2
Description: For quantitative detection of mouse Leptin receptor in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Mouse and Rat Leptin ELISA

GWB-95C312 ELISA_Kits Ask for price

Leptin ELISA Kit

GWB-B204DD 96 TESTS Ask for price

Leptin ELISA Kit

80968 96
EUR 335

Mouse Soluble Leptin Receptor ELISA kit

E01A25322 96T
EUR 700
Description: ELISA

Mouse Soluble Leptin Receptor ELISA kit

E03S0266-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Soluble Leptin Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Soluble Leptin Receptor ELISA kit

E03S0266-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Soluble Leptin Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Soluble Leptin Receptor ELISA kit

E03S0266-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Soluble Leptin Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Mouse LEPR (Leptin Receptor)

E-EL-M0749 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Mouse LEPR (Leptin Receptor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse LEPR (Leptin Receptor)

ELK2282 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Leptin Receptor from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Leptin(LEP)ELISA ki

QY-E20902 96T
EUR 433.2

Mouse Leptin Soluble Receptor,sLR ELISA Kit

CN-02514M1 96T
EUR 529.2

Mouse Leptin Soluble Receptor,sLR ELISA Kit

CN-02514M2 48T
EUR 349.2

Mouse Leptin Soluble Receptor(sLR)ELISA Kit  

GA-E0216MS-48T 48T
EUR 403.2

Mouse Leptin Soluble Receptor(sLR)ELISA Kit  

GA-E0216MS-96T 96T
EUR 640.8

Mouse Leptin Soluble Receptor(sLR)ELISA Kit

QY-E20901 96T
EUR 433.2

Mouse Leptin Soluble Receptor, sLR ELISA Kit

SELI-66068m 96 Tests
EUR 788.4

La protein chinasi Akt è cruciale per i risultati di promozione della crescita e anti-apoptotici della leptina nelle cellule di adenocarcinoma esofageo. Akt viene attivato attraverso percorsi dipendenti da Ras, Rho e cdc42. L’atorvastatina riduce l’attivazione di Akt indotta dalla leptina inibendo la prenilazione di piccole GTPasi. Ciò può chiarire sempre più la ridotta incidenza di adenocarcinoma esofageo nei consumatori di statine.

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