Livello di adiponectina, leptina e metalloproteinasi della matrice selezionate in pazienti in sovrappeso di sesso femminile con gonartrosi primaria

Level of Adiponectin, Leptin and Selected Matrix Metalloproteinases in Female Overweight Patients with Primary Gonarthrosis
L’obiettivo di questa ricerca era valutare i range di adiponectina (ACRP-30) e  leptina  nel siero e nel liquido sinoviale (SF) di donne obese con gonartrosi maggiore (GOA) e ricercare le loro correlazioni con la posizione scientifica e l’entità dell’OA biochimica biomarcatori: metalloproteinasi di matrice (MMP) 1, MMP-9 e inibitore tissutale della metalloproteinasi (TIMP-1). Il gruppo studiato era composto da 39 donne obese che hanno subito un intervento di artroplastica completa del ginocchio a causa di GOA.
Lo stadio di GOA è stato valutato in risposta ai punteggi Knee Society Rating (KSS), Ahlbäck e Kellgren-Lawrence (Ok-L). Erano stati prelevati sangue periferico e SF. Gli intervalli di biomarcatori scelti sono stati valutati utilizzando   kit ELISA . Lo stadio implicato di ACRP-30 nel siero valutato era di 8393,80 ng/mL e in SF, 774,33 ng/mL, il focus principale di  leptina  nel siero era 32.040,74 pg/mL e in SF, 27.332,12 pg/mL. Intervalli di  leptina  nel siero e SF correlati con l’indice di massa corporea (BMI), ( p  = 0,0005 e  p  = 0,0002, rispettivamente).
Gli intervalli di ACRP-30 nel siero sono stati correlati con i punteggi scientifici (Ahlbäck:  p  = 0,0214; Ok-L:  p  = 0,0146). ACRP-30 in SF correlato con ACRP-30 nel siero ( p  = 0,0003), tendeva a correlare negativamente con MMP-1 nel siero ( p  = 0,0598) e correlare positivamente con pro-MMP-1 in SF ( p  = 0,0600). Per concludere, questa ricerca conferma le correlazioni tra le concentrazioni di ciascuno,  leptina  e ACRP-30, valutando siero e SF. Nelle femmine obese, i   livelli di leptina migliorano con l’IMC e lo stadio sierico ACRP-30 migliora con ulteriori livelli superiori di GOA. Infine, la  leptina gli intervalli erano stati correlati con il focus sierico di TIMP-1, uno dei tanti marcatori biochimici di GOA.

Le cellule adipose pancreatiche delle persone con diabete di tipo 2 mostrano un ridotto esercizio adipogenico e lipolitico

I problemi di peso, in particolare l’accumulo di grassi viscerali, aumenteranno la possibilità di diabete di tipo 2 (T2D). Lo scopo di questa ricerca era di studiare l’influenza del diabete di tipo 2 sul deposito di grassi pancreatici. Sono stati raccolti cuscinetti di grasso pancreatico da 17 pazienti parzialmente pancreatectomizzati (PPP), preadipociti pancreatici isolati e differenziati in vitro. I malati sono stati raggruppati utilizzando HbA1c in normali tolleranti al glucosio (NGT), prediabetici (PD) e T2D. I profili del trascrittoma di preadipociti e adipociti sono stati valutati mediante RNAseq.
La sensibilità all’insulina è stata stimata quantificando la fosforilazione di AKT su western blot. La capacità lipogenica è stata valutata con Otaining rosa olio, esercizio lipolitico attraverso il lancio di acidi grassi. Gli elementi secreti sono stati misurati utilizzando  ELISA . La valutazione comparativa del trascrittoma di preadipociti e adipociti indica una sovraregolazione difettosa dei geni che governano l’adipogenesi (NR1H3), la lipogenesi (FASN, SCD, ELOVL6, FADS1) e la lipolisi (LIPE) durante la differenziazione delle cellule da T2D-PPP.
Inoltre, il rapporto tra  leptina /adiponectina mRNA era maggiore in T2D che in NGT-PPP. I preadipociti e gli adipociti di NGT-PPP erano più sensibili all’insulina rispetto alle cellule T2D-PPP per quanto riguarda la fosforilazione di AKT. L’accumulo di trigliceridi era paragonabile negli adipociti NGT e T2D. Indipendentemente da un’eccessiva espressione dei recettori NPR1 e NPR2 negli adipociti NGT e T2D, la lipolisi è stata stimolata da ANP 1,74 volte esclusivamente nelle cellule NGT.
Questa stimolazione è stata ulteriormente elevata dal dipiridamolo inibitore della PDE5 (3,09 volte). Il dipiridamolo e la forskolina hanno aumentato il recettore della lipolisi in modo indipendente di 1,88 volte e 1,48 volte, rispettivamente, esclusivamente nelle cellule NGT. In conclusione, lo stato metabolico influenza in modo persistente la differenziazione e la lipolisi degli adipociti pancreatici. Queste alterazioni potrebbero peggiorare l’evento di T2D.

Esercizio antiproliferativo dell’agonista del recettore del peptide-1 simile al glucagone sulla mammella correlata ai problemi di peso La maggior parte dei tumori: l’influenza sulla modulazione dell’espressione delle adipochine negli adipociti e nella maggior parte delle cellule tumorali

I problemi di peso sono legati al pericolo eccessivo e alla prognosi infausta della maggior parte dei tumori al seno (BC). I problemi di peso favoriscono la proliferazione delle cellule BC modulando la produzione di adipochine, insieme ad adiponectina (anti-neoplasticadipochina), leptina  (adipochina cancerogena) e mediatori dell’infiammazione.
Nell’ambito della ricerca attuale abbiamo studiato i risultati anti-proliferativi di liraglutide (LG; farmaco antidiabetico e per la riduzione del peso) su cellule BC umane MCF-7 coltivate in terreno condizionato da cellule staminali derivate da tessuto adiposo in sovrappeso (ADSCs-CM) e se o meno questo impatto è mediato dalla modulazione delle adipochine nelle ADSC e nella maggior parte delle cellule tumorali. La proliferazione è stata studiata utilizzando il controllo di vitalità AlamarBlue, il test di formazione di colonie e la valutazione del ciclo cellulare.
Gli intervalli e l’espressione delle adipochine e dei loro recettori sono stati saggiati utilizzando  ELISA  e RT-PCR. LG ha provocato l’inibizione del 48% della proliferazione MCF-7 in ADSCs-CM in sovrappeso, ha ridotto la formazione di colonie e ha indotto l’arresto della parte G0/G1. LG ha inoltre ridotto i gradi dei mediatori dell’infiammazione, soppresso l’espressione della  leptina , mentre elevati intervalli di mRNA di adiponectina e dei loro recettori nelle ADSC in sovrappeso e nella maggior parte delle cellule tumorali coltivate in ADCS-CM in sovrappeso.
In conclusione, LG potrebbe mitigare lo sviluppo delle cellule BC in argomenti in sovrappeso; per questo motivo può benissimo essere utilizzato per la prevenzione scientifica e/o il rimedio del BC in soggetti in sovrappeso. Potrebbe aiutare a migliorare i risultati del trattamento e ridurre il tasso di mortalità nei pazienti in sovrappeso con BC.
Livello di adiponectina, leptina e metalloproteinasi della matrice selezionate in pazienti in sovrappeso di sesso femminile con gonartrosi primaria

Aptasensor per la quantificazione della  leptina  tramite PCR Amplificazione di brevi DNA-aptameri

La quantificazione delle proteine ​​è storicamente effettuata mediante test di immunoassorbimento enzimatico ( ELISA ), che comporta lunghi tempi di preparazione. Per battere questo, i nuovi approcci utilizzano gli aptameri come scelta alternativa agli anticorpi. In questo documento, presentiamo un metodo nuovo di zecca per quantificare le proteine ​​con brevi aptameri di DNA mediante risposta a catena della polimerasi (PCR) che porta a istanze di protocollo più brevi con limiti di rilevamento relativamente migliorati.
La metodologia proposta presenta una nuova opzione per quantificare contemporaneamente sia la proteina obiettivo che i corrispondenti brevi aptameri di DNA, che ci consente inoltre di caratterizzare completamente l’efficienza degli aptasensori. La leptina umana   viene utilizzata come proteina obiettivo per convalidare questo sistema, poiché è considerato un biomarcatore vitale per la ricerca sull’obesità.
Nei nostri esperimenti, abbiamo raggiunto il limite inferiore di rilevamento di 100 pg/mL in meno di 2 ore, un limite influenzato dalla dissociazione fissata  dall’aptamero  leptinico, che può benissimo essere migliorato scegliendo un aptamero in più. Grazie al metodo semplice ed economico, questo sistema potrebbe essere impiegato per implementazioni Lab-On-Chip e per una rapida quantificazione “in loco” delle proteine.

La leptina  stimola Akt nella maggior parte delle cellule tumorali esofagee attraverso una serie di piccole GTPasi sensibili all’atorvastatina

I problemi di peso sono un problema pericoloso per l’esofago di Barrett e l’adenocarcinoma esofageo. Il tessuto adiposo secerne l’ormone  leptina . La leptina  è un problema di sviluppo per un certo numero di tipi di cellule, insieme alle cellule di Barrett e alle cellule di adenocarcinoma esofageo. Le statine sono correlate alla riduzione delle cariche dell’esofago di Barrett e della maggior parte dei tumori esofagei e mostrano risultati antitumorali in vitro. I meccanismi di tali risultati non saranno del tutto stabiliti.
Abbiamo ora esaminato le conseguenze della  leptina  e della statina liposolubile, atorvastatina, sulla segnalazione tramite proteine ​​​​leganti GTP monomeriche e Akt. La proliferazione e l’apoptosi sono state valutate nelle cellule OE33. L’esercizio di Akt è stato quantificato mediante ELISA cellulare   e test della chinasi in vitro. Particolari inibitori di piccole molecole e un assemblaggio dominante-negativo erano stati usati per ridurre l’esercizio di Akt. Piccole GTPasi erano state inibite utilizzando la trasfezione di plasmidi dominanti negativi, inibitori della prenilazione e pretrattamento con atorvastatina.
La leptina ha  stimolato l’esercizio di Akt e la proliferazione cellulare e ha inibito l’apoptosi indotta dalla camptotecina in un metodo Akt-sensibile. La leptina ha  indotto la fosforilazione di Dangerous e FOXO1 in un metodo Akt-sensibile. La leptina ha  attivato Ras, Rac, RhoA e cdc42. La trasfezione di plasmidi dominanti-negativi ha confermato che l’  attivazione di Akt indotta dalla leptina richiedeva Ras, RhoA cdc42 ma non Rac. L’atorvastatina ha inibito l’  attivazione indotta dalla leptina di Ras, RhoA, cdc42 e Akt. Il co-trattamento con mevalonato ha impedito questi risultati dell’atorvastatina.

Mouse Leptin ELISA kit

E03L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin ELISA Kit

E-90LPT 1 x 96 well plate
EUR 441

Mouse Leptin ELISA Kit

55R-1636 1 kit
EUR 550
Description: ELISA kit for detection of Leptin in the research laboratory

Mouse Leptin ELISA kit

55R-2027 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Mouse Leptin (LEP) ELISA Kit

20-abx154317
  • EUR 5374.00
  • EUR 2868.00
  • EUR 676.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Leptin (LEP) ELISA Kit

abx570297-96tests 96 tests
EUR 731

Mouse Leptin, Lep ELISA Kit

DEIA130 10 plates
EUR 2495
Description: Mouse Leptin ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant Leptin in a sandwich ELISA format within the range of 20-1,250 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay Leptin in approximately 1,000 ELISA plate wells.

Mouse Leptin(LEP)ELISA Kit        

GA-E0665MS-48T 48T
EUR 336

Mouse Leptin(LEP)ELISA Kit        

GA-E0665MS-96T 96T
EUR 534

Mouse Lep/ Leptin ELISA Kit

E0859Mo 1 Kit
EUR 546

Mouse Leptin (LEP) ELISA Kit

abx050143-96tests 96 tests
EUR 668

Mouse Leptin (LEP) ELISA Kit

abx051335-96tests 96 tests
EUR 754

Mouse Leptin (LEP) ELISA Kit

abx254349-96tests 96 tests
EUR 731

Mouse Leptin (LEP) ELISA Kit

DLR-LEP-Mu-48T 48T
EUR 489
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin (LEP) ELISA Kit

DLR-LEP-Mu-96T 96T
EUR 635
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin AssayMax ELISA Kit

EML2001-1 96 Well Plate
EUR 477

Mouse Leptin (LEP) ELISA Kit

RDR-LEP-Mu-48Tests 48 Tests
EUR 511

Mouse Leptin (LEP) ELISA Kit

RDR-LEP-Mu-96Tests 96 Tests
EUR 709

Mouse Leptin (LEP) ELISA Kit

RD-LEP-Mu-48Tests 48 Tests
EUR 489

Mouse Leptin (LEP) ELISA Kit

RD-LEP-Mu-96Tests 96 Tests
EUR 677

Mouse Leptin ELISA Kit(LEP)

RK00380 96 Tests
EUR 521

ELISA kit for Mouse Leptin

EK1237 96 tests
EUR 469
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Leptin in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin, LEP ELISA kit

CSB-E04650m-24T 1 plate of 24 wells
EUR 165
Description: Quantitative sandwich ELISA kit for measuring Mouse Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Leptin, LEP ELISA kit

1-CSB-E04650m
  • EUR 500.00
  • EUR 3402.00
  • EUR 1820.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Mouse Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-10x96wellstestplate 10x96-wells test plate
EUR 3213.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-1x48wellstestplate 1x48-wells test plate
EUR 352.25
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-1x96wellstestplate 1x96-wells test plate
EUR 460.36
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

SEA084Mu-5x96wellstestplate 5x96-wells test plate
EUR 1771.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit

4-SEA084Mu
  • EUR 3264.00
  • EUR 1722.00
  • EUR 461.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Leptin receptor ELISA Kit

LF-EK50834 1×96T
EUR 648

Mouse Leptin, LEP ELISA Kit

ELA-E0084m 96 Tests
EUR 865

Mouse Leptin Antibody ELISA kit

E03L0039-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin Antibody ELISA kit

E03L0039-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin Antibody ELISA kit

E03L0039-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Leptin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor ELISA kit

E03L0273-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor ELISA kit

E03L0273-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor ELISA kit

E03L0273-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin Receptor ELISA kit

55R-1638 1 kit
EUR 590
Description: ELISA kit for the detection of Leptin Receptor in the research laboratory

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 202

Human Leptin R(Leptin receptor) ELISA Kit

EH0217 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

ELISA kit for Mouse Leptin (LEP)

KTE71186-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Mouse Leptin (LEP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Leptin (LEP)

KTE71186-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Mouse Leptin (LEP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Leptin (LEP)

KTE71186-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Mouse Leptin (LEP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse LEP (Leptin)

ELK1161 1 plate of 96 wells
EUR 372
Description: A sandwich ELISA kit for detection of Leptin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

20-abx154318
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Leptin Receptor (LEPR) ELISA Kit

abx575468-96tests 96 tests
EUR 707

ELISA kit for Mouse LEP (Leptin)

E-EL-M0039 1 plate of 96 wells
EUR 377
Description: A sandwich ELISA kit for quantitative measurement of Mouse LEP (Leptin) in samples from Serum, Plasma, Cell supernatant

Mouse Lepr/ Leptin receptor ELISA Kit

E0860Mo 1 Kit
EUR 571

Mouse Leptin Receptor (LEPR) ELISA Kit

abx254357-96tests 96 tests
EUR 707

Mouse Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Mu-48T 48T
EUR 489
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin Receptor (LEPR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

DLR-LEPR-Mu-96T 96T
EUR 635
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin Receptor (LEPR) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Mu-48Tests 48 Tests
EUR 511

Mouse Leptin Receptor (LEPR) ELISA Kit

RDR-LEPR-Mu-96Tests 96 Tests
EUR 709

Mouse Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Mu-48Tests 48 Tests
EUR 489

Mouse Leptin Receptor (LEPR) ELISA Kit

RD-LEPR-Mu-96Tests 96 Tests
EUR 677

Leptin R ELISA Kit (Mouse) (OKBB00209)

OKBB00209 96 Tests
EUR 518
Description: Description of target: Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 5 pg/mL

ELISA kit for Mouse Leptin receptor

EK1229 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Leptin receptor in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-10x96wellstestplate 10x96-wells test plate
EUR 4391.16
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-1x48wellstestplate 1x48-wells test plate
EUR 449.27
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-1x96wellstestplate 1x96-wells test plate
EUR 598.96
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

SEA083Mu-5x96wellstestplate 5x96-wells test plate
EUR 2395.32
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin Receptor (LEPR) in serum, plasma, tissue homogenates and other biological fluids.

Mouse Leptin Receptor (LEPR) ELISA Kit

4-SEA083Mu
  • EUR 4442.00
  • EUR 2346.00
  • EUR 599.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Leptin Receptor (LEPR) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Mouse Leptin Receptor(LEPR)ELISA Kit

QY-E20899 96T
EUR 361

Mouse Leptin receptor(LEPR) ELISA kit

E03L0327-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor(LEPR) ELISA kit

E03L0327-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Leptin receptor(LEPR) ELISA kit

E03L0327-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Leptin receptor(LEPR) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Soluble Leptin Receptor ELISA kit

E03S0266-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Soluble Leptin Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Soluble Leptin Receptor ELISA kit

E03S0266-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Soluble Leptin Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Soluble Leptin Receptor ELISA kit

E03S0266-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Soluble Leptin Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Leptin-Mouse

PR27099 200 ug
EUR 191

Sheep Leptin ELISA Kit

ESL0045 96Tests
EUR 521

Human Leptin ELISA Kit

DEIA1165 96T
EUR 732
Description: The Human LEP ELISA kit is designed to detect and quantify the level of Human LEP in cell culture supernatant, serum, plasma and tissue. This kit is for research use only and not intended for diagnostic purposes.

Monkey Leptin ELISA Kit

EMKL0045 96Tests
EUR 521

Porcine Leptin ELISA Kit

EPL0045 96Tests
EUR 521

Rat Leptin ELISA Kit

ERL0045 96Tests
EUR 521

Rabbit Leptin ELISA Kit

ERTL0045 96Tests
EUR 521

Anserini Leptin ELISA Kit

EAL0045 96Tests
EUR 521

Goat Leptin ELISA Kit

EGTL0045 96Tests
EUR 521

Chicken Leptin ELISA Kit

ECKL0045 96Tests
EUR 521

Canine Leptin ELISA Kit

ECL0045 96Tests
EUR 521

Human Leptin ELISA Kit

EHL0045 96Tests
EUR 521

Bovine Leptin ELISA Kit

EBL0045 96Tests
EUR 521

Human Leptin ELISA Kit

RK00035 96 Tests
EUR 521

Sheep Leptin ELISA Kit

QY-E140030 96T
EUR 413

Leptin (human) ELISA Kit

K2191-100 100 assays
EUR 1017

Human Leptin ELISA kit

LF-EK50217 1×96T
EUR 648

Bovine Leptin ELISA Kit

ELA-E0084b 96 Tests
EUR 928

Canine Leptin ELISA Kit

ELA-E0084d 96 Tests
EUR 928

Goat Leptin ELISA Kit

ELA-E0084g 96 Tests
EUR 928

Porcine Leptin ELISA Kit

ELA-E0084p 96 Tests
EUR 928

Rat Leptin ELISA Kit

ELA-E0084r 96 Tests
EUR 886

Rabbit Leptin ELISA Kit

ELA-E0084Rb 96 Tests
EUR 928

Leptin (Human) ELISA Kit

E4653-100
EUR 729

Leptin (Rat) ELISA Kit

E4655-100
EUR 729

Leptin (human) ELISA Kit

K4777-100 100 assays
EUR 809
Description: Sensitive, Colorimetric Assay

Pig Leptin ELISA kit

E07L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Leptin ELISA kit

E07L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Leptin ELISA kit

E07L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Leptin ELISA kit

E08L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Leptin ELISA kit

E08L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Leptin ELISA kit

E08L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Leptin ELISA kit

E06L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Leptin ELISA kit

E06L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Leptin ELISA kit

E06L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit

E04L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit

E04L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Leptin ELISA kit

E04L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Leptin ELISA kit

E02L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

E01L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

E01L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA kit

E01L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Leptin ELISA kit

E09L0038-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Leptin ELISA kit

E09L0038-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Leptin ELISA kit

E09L0038-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Leptin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Leptin ELISA Kit

E-80LPT 1 x 96 well plate
EUR 405

Human Leptin ELISA kit

55R-1635 1 kit
EUR 476
Description: ELISA kit for the detection of Human Leptin in the research laboratory

Rat Leptin ELISA kit

55R-1953 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Bovine Leptin ELISA kit

55R-1959 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA kit

55R-1998 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Rabbit Leptin ELISA kit

55R-2006 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Porcine Leptin ELISA kit

55R-2032 96 tests
EUR 617
Description: ELISA Kit for detection of Leptin in the research laboratory

Human Leptin ELISA kit

55R-2265 96 tests
EUR 916
Description: ELISA Kit for detection of Leptin in the research laboratory

ELISA kit for Mouse LEPR (Leptin Receptor)

E-EL-M0749 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse LEPR (Leptin Receptor) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse LEPR (Leptin Receptor)

ELK2282 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Leptin Receptor from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Leptin Soluble Receptor(sLR)ELISA Kit  

GA-E0216MS-48T 48T
EUR 336

Mouse Leptin Soluble Receptor(sLR)ELISA Kit  

GA-E0216MS-96T 96T
EUR 534

Mouse Leptin ELISA Kit, 96 tests, Quantitative

100-130-LEM 1 Kit
EUR 712

Mouse Leptin Soluble Receptor(sLR)ELISA Kit

QY-E20901 96T
EUR 361

Leptin ELISA Kit (Mouse) : 96 Wells (OKAG00091)

OKAG00091 96 Wells
EUR 596
Description: Description of target: Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed:15899045, PubMed:16825198, PubMed:11373681, PubMed:12594516, PubMed:20620997). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) (PubMed:8589726, PubMed:10660043, PubMed:25383904, PubMed:25060689, PubMed:9732873, PubMed:12594516). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity) (PubMed:20620997, PubMed:11373681). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity) (PubMed:16825198, PubMed:20620997). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (By similarity). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed:16825198). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (Probable). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (PubMed:15899045). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4+CD25- T cells proliferation and reduces autophagy during TCR (T cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (PubMed:25060689).;Species reactivity: Mouse;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 20 pg/mL

Mouse Leptin Soluble Receptor,sLR ELISA Kit

CN-02514M1 96T
EUR 441

Mouse Leptin Soluble Receptor,sLR ELISA Kit

CN-02514M2 48T
EUR 291

Mouse Leptin Soluble Receptor, sLR ELISA Kit

SELI-66068m 96 Tests
EUR 657

Mouse Leptin(LEP)ELISA ki

QY-E20902 96T
EUR 361

Mouse Leptin (LEP) CLIA Kit

20-abx190439
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Leptin (LEP) CLIA Kit

abx195965-96tests 96 tests
EUR 825

Mouse Leptin (LEP)CLIA Kit

SCA084Mu-10x96wellstestplate 10x96-wells test plate
EUR 5804.88
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP)CLIA Kit

SCA084Mu-1x48wellstestplate 1x48-wells test plate
EUR 565.7
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP)CLIA Kit

SCA084Mu-1x96wellstestplate 1x96-wells test plate
EUR 765.28
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP)CLIA Kit

SCA084Mu-5x96wellstestplate 5x96-wells test plate
EUR 3143.76
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) CLIA Kit

4-SCA084Mu
  • EUR 5855.00
  • EUR 3144.00
  • EUR 766.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Mouse Leptin (LEP)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Recombinant Mouse Leptin

7-01144 200µg Ask for price

Recombinant Mouse Leptin

7-01145 1mg Ask for price

Recombinant Mouse Leptin

7-01146 5mg Ask for price

Recombinant Mouse Leptin

CG075-101 1mg
EUR 408

Recombinant Mouse Leptin

CG075-105 5mg
EUR 1252

Recombinant Mouse Leptin

CG075-200 200ug
EUR 293

Mouse Leptin Antibody

11431-05011 150 ug
EUR 217

Recombinant Mouse Leptin

P0191 100ug
EUR 522.36
Description: Recombinant Mouse protein for Leptin

rec Leptin (mouse)

H-5582.0200 0.2mg
EUR 162
Description: CAS# [1061787-61-8]

rec Leptin (mouse)

H-5582.1000 1.0mg
EUR 381
Description: CAS# [1061787-61-8]

Mouse Leptin protein

30R-1100 100 ug
EUR 397
Description: Purified recombinant Mouse Leptin protein

ELISA kit for Human Leptin

KET6026-48T 48T
EUR 202
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin

KET6026-5platesof96wells 5 plates of 96 wells
EUR 1077
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Leptin

KET6026-96T 96T
EUR 289
Description: Quantitative sandwich ELISA for measuring Human Leptin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

La protein chinasi Akt è cruciale per i risultati di promozione della crescita e anti-apoptotici della leptina nelle cellule di adenocarcinoma esofageo. Akt viene attivato attraverso percorsi dipendenti da Ras, Rho e cdc42. L’atorvastatina riduce l’attivazione di Akt indotta dalla leptina inibendo la prenilazione di piccole GTPasi. Ciò può chiarire sempre più la ridotta incidenza di adenocarcinoma esofageo nei consumatori di statine.

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