Proteinase K

For in vitro use only!

Definition of unit:

A unit is the amount of enzyme that liberates at 37 ° C in 1 min as many amino acids and haemoglobin peptides positive for folin as 1 μmol of tyrosine.

Shipping: shipped in gel packs

Storage conditions:

  • Store at -20 ° C
  • Avoid freeze/thaw cycles

Shelf life: 12 months.

Molecular weight: 28.9 kDa

CAS #: 39450-01-6

EC number: 254-457-8

Purity:

free of RNases, DNases and Exonucleases

Form:

Proteinase K solution in 10 mM Tris-HCl, 1 mM Ca (H3C2O2) 2, Glycerol 10% (v / v), pH 7.8 (22 ° C)

Concentration: 20mg / ml

Exercise: > 600 units / ml

Applications:

Protein digestion during DNA and RNA preparation.

Description:

Proteinase K is a serine protease that exhibits very broad cleavage specificity. The protein with a molecular weight of 28.9 kDa cleaves the peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Protein K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide pH range (4-12.5).

Proteinase K is a very active and stable protease with low shear specificity. The enzyme belongs to the group of serine proteases related to subtilisin and is strongly inhibited by PMSF. In the presence of 0.5-1% SDS, proteinase K inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of proteinase K during cell lysis allows the isolation of intact high molecular weight nucleic acids.

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