Ormoni metabolici del latte umano: metodi analitici e attuali conoscenze

| GianfrancoGianfranco | 0 Comment
Human Milk Metabolic Hormones: Analytical Methods and Current Understanding
Il latte umano (HM)  contiene un’ampia selezione di ormoni peptidici insieme a  leptina e adiponectina, che sono coinvolti nella regolazione del progresso e del miglioramento del bambino. Questi importanti ormoni possono svolgere una funzione essenziale nella regolazione della riprogrammazione metabolica del neonato. Tuttavia, la ricerca sugli ormoni HM è scarsa ed eterogenea per quanto riguarda il disegno della ricerca, l’assortimento dei modelli, le strategie di preparazione e valutazione.
Questa panoramica ha menzionato i vincoli della valutazione dell’ormone HM evidenziando le lacune nelle fasi pre-analitiche e analitiche. Le strategie utilizzate per quantificare gli ormoni metabolici HM ( leptina , adiponectina, grelina, insulina, obostatina, resistina e apelina) possono essere etichettate come immunodosaggio, immunosensore e cromatografia. Le strategie di dosaggio immunologico ( ELISA  e RIA) sono state utilizzate prevalentemente nella misurazione di quegli ormoni HM. I parametri di validità relativa della valutazione degli ormoni HM sono talvolta ignorati nelle pubblicazioni, indipendentemente dalla complessità e dalle variazioni della matrice HM rispetto a quella del plasma e delle urine.
Per questo motivo, gli studi applicabili sui parametri di convalida della metodologia e della strumentazione sono essenziali per misurazioni corrette e, di conseguenza, per una migliore comprensione degli ormoni metabolici HM e delle loro influenze sugli esiti dei bambini.

La leptina derivata dal tessuto adiposo pericardico   promuove l’apoptosi miocardica nei ratti sovrappeso indotti da un piano di riduzione del peso tramite Janus chinasi 2/Specie reattive dell’ossigeno/Na+/Va bene+-ATPasi Via di segnalazione

Contesto I meccanismi fisiopatologici alla base degli aggiustamenti strutturali cardiaci e mirati nei problemi di peso sono avanzati e collegati alle adipocitochine lanciate dal tessuto adiposo pericardico (PAT) e dall’apoptosi dei cardiomiociti. Sebbene la  leptina  sia coinvolta in varie situazioni patologiche, la sua funzione nel movimento paracrino del tessuto adiposo pericardico sull’apoptosi miocardica rimane sconosciuta. Questa ricerca è stata progettata per ricercare la funzione della leptina derivata da PAT   sull’apoptosi miocardica in ratti in sovrappeso indotti da dieta ricca di grassi.
Strategie e risultati Hearts sono stati allontanati da ratti Wistar in sovrappeso magri o ad alto contenuto di grassi per la ricerca sulla trasformazione del miocardio. I ratti in sovrappeso avevano una costruzione miocardica irregolare, disfunzione diastolica, apoptosi cardiaca drasticamente elevata, fibrosi cardiaca aumentata e stadio di stress ossidativo elevato. L’ELISA ha  rilevato uno  stadio considerevolmente maggiore dello stadio di leptina circolante  nella PAT di ratti in sovrappeso, ma non magri. Western blot e analisi immunoistochimiche hanno dimostrato un’elevata   densità del recettore della leptina nei cuori in sovrappeso. I cardiomioblasti H9c2, dopo essere stati scoperti in un mezzo condizionato con PAT di ratti in sovrappeso, hanno mostrato un’apoptosi mediata da specie reattive dell’ossigeno pronunciata, che è stata parzialmente invertita dalla  leptina antagonista.
Inoltre, la  leptina  derivata dalla PAT di ratti sovrappeso ha inibito l’esercizio di Na + /Okay + -ATPasi delle cellule H9c2 stimolando specie reattive dell’ossigeno, attivando così l’apoptosi calcio-dipendente. Il pretrattamento con particolari inibitori ha rivelato che le vie di segnalazione Janus chinasi 2/segno e attivatore della trascrizione Tre e fosfoinositide 3-chinasi/proteina chinasi B sono state coinvolte  nell’apoptosi miocardica indotta dalla leptina . Conclusioni La leptina derivata da PAT   induce apoptosi miocardica in ratti sovrappeso indotti da dieta ricca di grassi attraverso l’attivazione del trasduttore Janus chinasi 2/segno e dell’attivatore della via di segnalazione delle specie trascrizionali 3/reattive dell’ossigeno e inibendo il suo Na + a valle/Ok + -Esercizio ATPasi.

Potenziale modificante la malattia di metformina e alendronato in un manichino sperimentale di topo di osteoartrite

L’osteoartrite (OA) è la più tipica malattia degenerativa articolare che causa danni progressivi della cartilagine e dell’osso subcondrale, irritazione sinoviale e dolore estremo. Indipendentemente dagli aggiustamenti patomorfologici avanzati che si verificano nell’OA, la strategia per tipi completamente diversi di OA è standardizzata.
I risultati mondiali del rimedio farmacologico non dovrebbero essere superabili. Pertanto, questa ricerca mirava a scoprire i risultati di metformina, alendronato e la loro miscela sul miglioramento e lo sviluppo dell’OA nei topi con osteoartrite indotta da collagenasi (CIOA). Topi femminili ICR (CD-2) sono stati randomizzati a 5 squadre: gruppo di gestione, CIOA non trattato, CIOA + metformina, CIOA + alendronato e CIOA + metformina + alendronato. L’OA è stata indotta dall’iniezione intra-articolare (ia) di collagenasi. Il fenotipo dell’OA è stato analizzato mediante citometria del movimento (differenziazione delle cellule del midollo osseo) ,  ELISA  (gamme sieriche delle adipochine  leptina  e resistina) e istologia (aggiustamenti patologici dell’articolazione del ginocchio).
Il rimedio con metformina, alendronato o la loro miscela ha inibito l’espressione di RANK e RANKL su osteoblasti e osteoclasti ottenuti mediante coltivazione ex vivo di cellule del midollo osseo in mezzi di mineralizzazione o osteoclastogenici. Inoltre, il rimedio a base di metformina è stato efficace per l’attenuazione della differenziazione dei fibroblasti, ma non delle cellule staminali mesenchimali (MSC), mentre l’alendronato ha avuto un impatto inverso. La miscela di metformina e alendronato ha avuto un impatto soppressivo sulla differenziazione di ciascuna MSC e fibroblasti. Rimedio con metformina, alendronato e la loro miscela ha diminuito le concentrazioni sieriche di  leptina  e resistina all’interno della sezione persistente dell’artrite.
L’esame istopatologico ha confermato che, contrariamente al gruppo CIOA non trattato (valutazione OA 9), le squadre trattate con metformina (valutazione OA 4) o alendronato (valutazione OA 6) avevano punteggi di diminuzione per gli aggiustamenti della cartilagine. La metformina mescolata con alendronato ha notevolmente ridotto il grado di degenerazione della cartilagine (classificazione OA 2), suggerendo che questa miscela può essere una strategia utile per il trattamento dei malati di OA.

Campione di espressione della  leptina  e dei suoi recettori nell’endometrio dell’endometrio La maggior parte dei tumori

L’identificazione di nuovi marcatori molecolari e l’eventualità della maggior parte dei metodi di trattamento del cancro sono essenziali poiché l’incidenza della maggior parte dei tumori continua ad essere molto eccessiva. I problemi di peso possono contribuire allo sviluppo della maggior parte dei tumori, insieme alla maggior parte dei tumori dell’endometrio. Gli adipociti secernono  leptina , che, quando è in uno stadio eccessivo, è correlata a un rischio elevato della maggior parte dei tumori. L’obiettivo di questa ricerca era scoprire il profilo di espressione dei  geni correlati alla leptina all’interno dei campioni di tessuto endometriale e del sangue completo dei malati.
I materiali di ricerca includevano campioni di tessuto e sangue prelevato da 30 pazienti con maggior parte dei tumori dell’endometrio e 30 senza la maggior parte dei tumori. I microarray sono stati utilizzati per valutare il profilo di espressione dei  geni correlati alla leptina . Quindi, l’espressione della  leptina  (LEP), del   recettore della leptina (LEPR),   del trascritto sovrapposto del recettore della leptina (LEPROT) e   del trascritto sovrapposto del recettore della leptina simile 1 (LEPROTL1) è stata decisa dalla risposta alla trascrizione inversa quantitativa in tempo reale (RT-qPCR). ). Il  focus della leptina sierica è  stato valutato utilizzando il saggio di immunoassorbimento enzimatico ( ELISA ).
La leptina  e i suoi recettori sono stati sovraespressi ciascuno sugli intervalli di mRNA e proteine. Inoltre, ci sono state solide correlazioni ottimistiche tra i   livelli di leptina e l’indice di massa fisica (BMI) della persona interessata. Livelli elevati di  leptina  e dei suoi recettori potrebbero probabilmente contribuire allo sviluppo della maggior parte dei tumori dell’endometrio. Queste osservazioni potrebbero anche essere utili nella progettazione di metodi di rimedio per la maggior parte dei tumori dell’endometrio.
Ormoni metabolici del latte umano: metodi analitici e attuali conoscenze

Effetto della  leptina  sui fibroblasti del legamento parodontale durante la pressione meccanica

Il rimedio ortodontico per correggere le malocclusioni dentali determina la formazione di zone di deformazione all’interno del legamento parodontale che portano a una risposta infiammatoria sterile, che è mediata dai fibroblasti del legamento parodontale (PDLF). I  livelli di leptina sono elevati nei problemi di peso e nelle risposte infiammatorie persistenti. In considerazione della crescente varietà di pazienti ortodontici con queste situazioni, le informazioni sui risultati sui rimedi ortodontici sono di notevole rilevanza medica.  Tuttavia, un effetto fattibile della  leptina sul profilo di espressione del PDLF durante la pressione meccanica ortodontica simulata non è stato studiato.
In questa ricerca, sono stati scoperti PDLF a pressione meccanica con o senza  concentrazioni di leptina completamente diverse  . L’espressione genica e proteica di componenti proinfiammatorie e di rimodellamento osseo è stata analizzata con RT-qPCR, Western-blot ed  ELISA . La valutazione mirata dell’osteoclastogenesi indotta da PDLF è stata analizzata mediante colorazione TRAP (fosfatasi acida tartrato-resistente) in cocoltura con macrofagi umani. L’aumento indotto dal ceppo di componenti proinfiammatorie è stato inoltre elevato con il   farmaco a base di leptina .
Il PDLF ha considerevolmente elevato l’espressione di RANKL (attivatore del recettore del ligando NF-kB) dopo la compressione, mentre l’osteoprotegerina era downregolata. È  stato dimostrato un ulteriore  impatto della leptina per RANKL oltre che per la successiva osteoclastogenesi in coculture dopo la colorazione TRAP.

Pig Leptin (LEP) ELISA Kit
4-SEA084Po
  • EUR 6178.80
  • EUR 3264.00
  • EUR 819.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Leptin (LEP) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Pig LEP/ Leptin ELISA Kit
E0118Pi 1 Kit
EUR 860.4

Guinea pig Leptin (LEP) ELISA Kit
abx354786-96tests 96 tests
EUR 904.8

ELISA kit for Pig Leptin (LEP)
KTE80141-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Pig Leptin (LEP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Pig Leptin (LEP)
KTE80141-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Pig Leptin (LEP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Pig Leptin (LEP)
KTE80141-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Pig Leptin (LEP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Pig LEP (Leptin)
ELK5723 1 plate of 96 wells
EUR 631.2
Description: A sandwich ELISA kit for detection of Leptin from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Pig Leptin (LEP) CLIA Kit
20-abx190809
  • EUR 11090.40
  • EUR 5904.00
  • EUR 1356.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Pig Leptin (LEP)CLIA Kit
SCA084Po-10x96wellstestplate 10x96-wells test plate
EUR 8096.83
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Leptin (LEP) in serum, plasma, tissue homogenates and other biological fluids.

Pig Leptin (LEP)CLIA Kit
SCA084Po-1x48wellstestplate 1x48-wells test plate
EUR 771.97
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Leptin (LEP) in serum, plasma, tissue homogenates and other biological fluids.

Pig Leptin (LEP)CLIA Kit
SCA084Po-1x96wellstestplate 1x96-wells test plate
EUR 1051.39
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Leptin (LEP) in serum, plasma, tissue homogenates and other biological fluids.

Pig Leptin (LEP)CLIA Kit
SCA084Po-5x96wellstestplate 5x96-wells test plate
EUR 4371.26
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Pig Leptin (LEP) in serum, plasma, tissue homogenates and other biological fluids.

Pig Leptin (LEP) CLIA Kit
4-SCA084Po
  • EUR 8157.60
  • EUR 4371.60
  • EUR 1052.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Pig Leptin (LEP)Serum, plasma, tissue homogenates and other biological fluids

Pig Leptin (LEP) Protein
20-abx654162
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Leptin (LEP) ELISA Kit
abx255813-96tests 96 tests
EUR 801.6

Rat Leptin (LEP) ELISA Kit
20-abx155779
  • EUR 6448.80
  • EUR 3441.60
  • EUR 811.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Leptin (LEP) ELISA Kit
20-abx150162
  • EUR 9174.00
  • EUR 4888.80
  • EUR 1130.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Dog Leptin (LEP) ELISA Kit
20-abx150273
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Dog Leptin(LEP) ELISA kit
CSB-EL012870DO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Dog Leptin (LEP) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Dog Leptin(LEP) ELISA kit
1-CSB-EL012870DO
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Dog Leptin(LEP) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Leptin,LEP ELISA kit
CN-01503R1 96T
EUR 530.4

Rat Leptin,LEP ELISA kit
CN-01503R2 48T
EUR 351.6

Rat Leptin (LEP) ELISA Kit
DLR-LEP-Ra-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leptin (LEP) ELISA Kit
DLR-LEP-Ra-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leptin, LEP ELISA kit
CSB-E07433r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Leptin, LEP in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Leptin, LEP ELISA kit
1-CSB-E07433r
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Leptin, LEP in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Leptin (LEP) ELISA Kit
RDR-LEP-Ra-48Tests 48 Tests
EUR 640.8

Rat Leptin (LEP) ELISA Kit
RDR-LEP-Ra-96Tests 96 Tests
EUR 890.4

Rat Leptin (LEP) ELISA Kit
RD-LEP-Ra-48Tests 48 Tests
EUR 613.2

Rat Leptin (LEP) ELISA Kit
RD-LEP-Ra-96Tests 96 Tests
EUR 850.8

Dog Leptin (LEP) ELISA Kit
SEA084Ca-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Leptin (LEP) in serum, plasma and other biological fluids.

Dog Leptin (LEP) ELISA Kit
SEA084Ca-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Leptin (LEP) in serum, plasma and other biological fluids.

Dog Leptin (LEP) ELISA Kit
SEA084Ca-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Leptin (LEP) in serum, plasma and other biological fluids.

Dog Leptin (LEP) ELISA Kit
SEA084Ca-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Leptin (LEP) in serum, plasma and other biological fluids.

Dog Leptin (LEP) ELISA Kit
4-SEA084Ca
  • EUR 5895.60
  • EUR 3114.00
  • EUR 786.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Dog Leptin (LEP) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Leptin (LEP) ELISA Kit
SEA084Ra-10x96wellstestplate 10x96-wells test plate
EUR 3855.67
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit
SEA084Ra-1x48wellstestplate 1x48-wells test plate
EUR 422.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit
SEA084Ra-1x96wellstestplate 1x96-wells test plate
EUR 552.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit
SEA084Ra-5x96wellstestplate 5x96-wells test plate
EUR 2125.94
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Rat Leptin (LEP) ELISA Kit
4-SEA084Ra
  • EUR 3916.80
  • EUR 2066.40
  • EUR 553.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Leptin (LEP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Leptin (LEP) ELISA Kit
RD-LEP-Ra-48T 48T
EUR 443.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Rat Leptin (LEP) ELISA Kit
RD-LEP-Ra-96T 96T
EUR 633.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Rat Leptin,LEP ELISA kit
YLA0116RA-48T 48T
EUR 465

Rat Leptin,LEP ELISA kit
YLA0116RA-96T 96T
EUR 600

Rat Leptin (LEP) ELISA Kit
RDR-LEP-Ra-48T 48T
EUR 465.47
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Rat Leptin (LEP) ELISA Kit
RDR-LEP-Ra-96T 96T
EUR 664.97
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Goat Leptin (LEP) ELISA Kit
abx570521-96tests 96 tests
EUR 1093.2

Goat Leptin (LEP) ELISA Kit
DLR-LEP-g-48T 48T
EUR 667.2
Description: A sandwich quantitative ELISA assay kit for detection of Goat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Leptin (LEP) ELISA Kit
DLR-LEP-g-96T 96T
EUR 873.6
Description: A sandwich quantitative ELISA assay kit for detection of Goat Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Leptin, LEP ELISA kit
CSB-E12834G-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Goat Leptin, LEP in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Goat Leptin, LEP ELISA kit
1-CSB-E12834G
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Goat Leptin, LEP in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Goat Leptin (LEP) ELISA Kit
RDR-LEP-g-48Tests 48 Tests
EUR 709.2

Goat Leptin (LEP) ELISA Kit
RDR-LEP-g-96Tests 96 Tests
EUR 987.6

Goat Leptin(LEP) ELISA Kit
QY-E140081 96T
EUR 495.6

Goat Leptin (LEP) ELISA Kit
RD-LEP-g-48Tests 48 Tests
EUR 679.2

Goat Leptin (LEP) ELISA Kit
RD-LEP-g-96Tests 96 Tests
EUR 944.4

Goat Leptin (LEP) ELISA Kit
RD-LEP-g-48T 48T
EUR 495.8
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Goat Leptin (LEP) ELISA Kit
RD-LEP-g-96T 96T
EUR 708.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

GOAT Leptin(LEP) ELISA Kit
YLA0033GO-48T 48T
EUR 465

GOAT Leptin(LEP) ELISA Kit
YLA0033GO-96T 96T
EUR 600

Fish leptin(LEP) ELISA Kit
YLA0022FI-48T 48T
EUR 547.5

Fish leptin(LEP) ELISA Kit
YLA0022FI-96T 96T
EUR 637.5

Goat Leptin (LEP) ELISA Kit
RDR-LEP-g-48T 48T
EUR 520.59
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Goat Leptin (LEP) ELISA Kit
RDR-LEP-g-96T 96T
EUR 743.72
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Leptin (LEP) ELISA Kit
20-abx152192
  • EUR 6448.80
  • EUR 3441.60
  • EUR 811.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Leptin (LEP) ELISA Kit
abx254349-96tests 96 tests
EUR 877.2

Human Leptin (LEP) ELISA Kit
abx251497-96tests 96 tests
EUR 801.6

Mouse Leptin (LEP) ELISA Kit
20-abx154317
  • EUR 6448.80
  • EUR 3441.60
  • EUR 811.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Horse Leptin (LEP) ELISA Kit
20-abx150465
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Leptin,LEP ELISA kit
201-12-1560 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Leptin (LEP) ELISA Kit
abx050142-96tests 96 tests
EUR 801.6

Mouse Leptin (LEP) ELISA Kit
abx050143-96tests 96 tests
EUR 801.6

Human Leptin (LEP) ELISA Kit
abx051330-96tests 96 tests
EUR 801.6

Mouse Leptin (LEP) ELISA Kit
abx051335-96tests 96 tests
EUR 904.8

Mouse Leptin (LEP) ELISA Kit
abx570297-96tests 96 tests
EUR 877.2

Sheep Leptin (LEP) ELISA Kit
20-abx585152
  • EUR 9174.00
  • EUR 4888.80
  • EUR 1130.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Leptin (LEP) ELISA Kit
abx570296-96tests 96 tests
EUR 801.6

Human Leptin, LEP ELISA kit
CSB-E04649h-24T 1 plate of 24 wells
EUR 198
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Leptin, LEP ELISA kit
1-CSB-E04649h
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Leptin, LEP ELISA kit
CSB-E04650m-24T 1 plate of 24 wells
EUR 198
Description: Quantitative sandwich ELISA kit for measuring Mouse Leptin, LEP in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Leptin, LEP ELISA kit
1-CSB-E04650m
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Mouse Leptin, LEP in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Leptin, Lep ELISA Kit
DEIA130 10 plates
EUR 2994
Description: Mouse Leptin ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant Leptin in a sandwich ELISA format within the range of 20-1,250 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay Leptin in approximately 1,000 ELISA plate wells.

Human Leptin, LEP ELISA Kit
DEIA167 96T
EUR 888
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitative measurement of Human leptin in serum, plasma and other biological fluids.

Horse Leptin(LEP) ELISA kit
CSB-EL012870HO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativecompetitive ELISA kit for measuring Horse Leptin (LEP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Horse Leptin(LEP) ELISA kit
1-CSB-EL012870HO
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Horse Leptin(LEP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Sheep Leptin(LEP) ELISA kit
CSB-EL012870SH-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Sheep Leptin (LEP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Sheep Leptin(LEP) ELISA kit
1-CSB-EL012870SH
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Sheep Leptin(LEP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Leptin,LEP ELISA kit
CN-03239H1 96T
EUR 530.4

Human Leptin,LEP ELISA kit
CN-03239H2 48T
EUR 351.6

Human Leptin (LEP) ELISA Kit
DLR-LEP-Hu-48T 48T
EUR 470.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leptin (LEP) ELISA Kit
DLR-LEP-Hu-96T 96T
EUR 602.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin (LEP) ELISA Kit
DLR-LEP-Mu-48T 48T
EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin (LEP) ELISA Kit
DLR-LEP-Mu-96T 96T
EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Leptin(LEP)ELISA Kit 　 　 　 　
GA-E0665MS-48T 48T
EUR 403.2

Mouse Leptin(LEP)ELISA Kit 　 　 　 　
GA-E0665MS-96T 96T
EUR 640.8

Human Leptin(LEP)ELISA Kit
GA-E1576HM-48T 48T
EUR 346.8

Human Leptin(LEP)ELISA Kit
GA-E1576HM-96T 96T
EUR 559.2

Mouse Leptin, LEP ELISA Kit
ELA-E0084m 96 Tests
EUR 1038

Human Leptin (LEP) ELISA Kit
RDR-LEP-Hu-48Tests 48 Tests
EUR 475.2

Human Leptin (LEP) ELISA Kit
RDR-LEP-Hu-96Tests 96 Tests
EUR 654

Mouse Leptin (LEP) ELISA Kit
RDR-LEP-Mu-48Tests 48 Tests
EUR 613.2

Mouse Leptin (LEP) ELISA Kit
RDR-LEP-Mu-96Tests 96 Tests
EUR 850.8

Human Leptin(LEP)ELISA Kit
QY-E01495 96T
EUR 433.2

Human Leptin (LEP) ELISA Kit
RD-LEP-Hu-48Tests 48 Tests
EUR 456

Human Leptin (LEP) ELISA Kit
RD-LEP-Hu-96Tests 96 Tests
EUR 625.2

Mouse Leptin (LEP) ELISA Kit
RD-LEP-Mu-48Tests 48 Tests
EUR 586.8

Mouse Leptin (LEP) ELISA Kit
RD-LEP-Mu-96Tests 96 Tests
EUR 812.4

Horse Leptin (LEP) ELISA Kit
SEA084Eq-10x96wellstestplate 10x96-wells test plate
EUR 6259
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Leptin (LEP) in serum, plasma and other biological fluids.

Horse Leptin (LEP) ELISA Kit
SEA084Eq-1x48wellstestplate 1x48-wells test plate
EUR 620.63
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Leptin (LEP) in serum, plasma and other biological fluids.

Horse Leptin (LEP) ELISA Kit
SEA084Eq-1x96wellstestplate 1x96-wells test plate
EUR 835.18
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Leptin (LEP) in serum, plasma and other biological fluids.

Horse Leptin (LEP) ELISA Kit
SEA084Eq-5x96wellstestplate 5x96-wells test plate
EUR 3398.29
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse Leptin (LEP) in serum, plasma and other biological fluids.

Horse Leptin (LEP) ELISA Kit
4-SEA084Eq
  • EUR 6319.20
  • EUR 3338.40
  • EUR 835.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Horse Leptin (LEP) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Leptin (LEP) ELISA Kit
SEA084Hu-10x96wellstestplate 10x96-wells test plate
EUR 3855.67
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
SEA084Hu-1x48wellstestplate 1x48-wells test plate
EUR 422.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
SEA084Hu-1x96wellstestplate 1x96-wells test plate
EUR 552.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
SEA084Hu-5x96wellstestplate 5x96-wells test plate
EUR 2125.94
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Leptin (LEP) in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Leptin (LEP) ELISA Kit
4-SEA084Hu
  • EUR 3916.80
  • EUR 2066.40
  • EUR 553.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Leptin (LEP) in samples from serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Leptin (LEP) ELISA Kit
SEA084Mu-10x96wellstestplate 10x96-wells test plate
EUR 3855.67
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit
SEA084Mu-1x48wellstestplate 1x48-wells test plate
EUR 422.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit
SEA084Mu-1x96wellstestplate 1x96-wells test plate
EUR 552.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit
SEA084Mu-5x96wellstestplate 5x96-wells test plate
EUR 2125.94
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Leptin (LEP) ELISA Kit
4-SEA084Mu
  • EUR 3916.80
  • EUR 2066.40
  • EUR 553.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Sheep Leptin (LEP) ELISA Kit
SEA084Ov-10x96wellstestplate 10x96-wells test plate
EUR 6541.74
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Sheep Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sheep Leptin (LEP) ELISA Kit
SEA084Ov-1x48wellstestplate 1x48-wells test plate
EUR 643.91
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Sheep Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sheep Leptin (LEP) ELISA Kit
SEA084Ov-1x96wellstestplate 1x96-wells test plate
EUR 868.44
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Sheep Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sheep Leptin (LEP) ELISA Kit
SEA084Ov-5x96wellstestplate 5x96-wells test plate
EUR 3547.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Sheep Leptin (LEP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sheep Leptin (LEP) ELISA Kit
4-SEA084Ov
  • EUR 6602.40
  • EUR 3488.40
  • EUR 868.80
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Sheep Leptin (LEP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Leptin (LEP) ELISA Kit
RD-LEP-Hu-48T 48T
EUR 317.3
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Leptin (LEP) ELISA Kit
RD-LEP-Hu-96T 96T
EUR 453.3
Description: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Mouse Leptin (LEP) ELISA Kit
RD-LEP-Mu-48T 48T
EUR 422.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Mouse Leptin (LEP) ELISA Kit
RD-LEP-Mu-96T 96T
EUR 603.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Mouse Leptin,LEP ELISA kit 　 　 　 　
YLA0175MO-48T 48T
EUR 465

Mouse Leptin,LEP ELISA kit 　 　 　 　
YLA0175MO-96T 96T
EUR 600

Horse Leptin,LEP ELISA kit
YLA0052HO-48T 48T
EUR 525

Horse Leptin,LEP ELISA kit
YLA0052HO-96T 96T
EUR 645

Sheep Leptin(LEP)ELISA Kit
YLA0086SH-48T 48T
EUR 540

I nostri risultati suggeriscono che concentrazioni elevate di leptina, come avviene nei pazienti in sovrappeso, potrebbero influenzare il movimento dei denti ortodontici. In particolare, si può presumere che l’elevata espressione di componenti proinfiammatorie e RANKL oltre all’elevata osteoclastogenesi acceleri il riassorbimento osseo e quindi il tasso di movimento dei denti ortodontici all’interno del rimedio ortodontico di chi soffre di sovrappeso.

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